Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8565 | 3' | -51.9 | NC_002169.1 | + | 78017 | 0.66 | 0.980729 |
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Target: 5'- cGACGcGAcCUcGaauaCGUGCGUGCCgaUCCCg -3' miRNA: 3'- -CUGUaUUuGA-Cg---GUACGCACGG--AGGG- -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 8553 | 0.66 | 0.97842 |
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Target: 5'- gGugGUGugauAGCUGCCGccgGUucgccuaaGUGCCUCUCg -3' miRNA: 3'- -CugUAU----UUGACGGUa--CG--------CACGGAGGG- -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 55977 | 0.66 | 0.9732 |
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Target: 5'- cGGCGUua--UGCCGUGCGUGUCg--- -3' miRNA: 3'- -CUGUAuuugACGGUACGCACGGaggg -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 87257 | 0.66 | 0.9732 |
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Target: 5'- aGCGUuauuCUGUCAaugGCGaGCCUCCUg -3' miRNA: 3'- cUGUAuuu-GACGGUa--CGCaCGGAGGG- -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 63299 | 0.72 | 0.798856 |
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Target: 5'- cGGCGUcuaGAGCUGCgGcgcuUGCGUGgCCUgCCCg -3' miRNA: 3'- -CUGUA---UUUGACGgU----ACGCAC-GGA-GGG- -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 109726 | 0.73 | 0.729762 |
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Target: 5'- gGACAUGAGCUGCgA-GCaaUGCCUCUCc -3' miRNA: 3'- -CUGUAUUUGACGgUaCGc-ACGGAGGG- -5' |
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| 8565 | 3' | -51.9 | NC_002169.1 | + | 84034 | 1.13 | 0.002917 |
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Target: 5'- aGACAUAAACUGCCAUGCGUGCCUCCCg -3' miRNA: 3'- -CUGUAUUUGACGGUACGCACGGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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