Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 857 | 5' | -54.9 | NC_000898.1 | + | 92006 | 0.66 | 0.961902 |
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Target: 5'- -----aCCUUUUCUCCGC-CuUCCCGa -3' miRNA: 3'- ccugcaGGGAAAGAGGCGuGuAGGGC- -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 75682 | 0.66 | 0.94814 |
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Target: 5'- aGAUGUCgaauucucaaucaaCUUUCUCCcuuGCuACAUCCCGg -3' miRNA: 3'- cCUGCAGg-------------GAAAGAGG---CG-UGUAGGGC- -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 5552 | 0.68 | 0.904455 |
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Target: 5'- cGACGUCCCUccccgCCGCuCG-CCCGu -3' miRNA: 3'- cCUGCAGGGAaaga-GGCGuGUaGGGC- -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 158720 | 0.68 | 0.904455 |
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Target: 5'- cGACGUCCCUccccgCCGCuCG-CCCGu -3' miRNA: 3'- cCUGCAGGGAaaga-GGCGuGUaGGGC- -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 57670 | 0.7 | 0.820134 |
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Target: 5'- gGGAUGUCCaUUUCUUCGagcuuuuuuugaaGCAUCCCu -3' miRNA: 3'- -CCUGCAGGgAAAGAGGCg------------UGUAGGGc -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 2246 | 0.73 | 0.662489 |
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Target: 5'- gGGACGUCCCcggUUgUCa-CACGUCCCu -3' miRNA: 3'- -CCUGCAGGGa--AAgAGgcGUGUAGGGc -5' |
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| 857 | 5' | -54.9 | NC_000898.1 | + | 155413 | 0.73 | 0.662489 |
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Target: 5'- gGGACGUCCCcggUUgUCa-CACGUCCCu -3' miRNA: 3'- -CCUGCAGGGa--AAgAGgcGUGUAGGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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