Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8571 | 3' | -45.3 | NC_002169.1 | + | 74477 | 0.66 | 0.999995 |
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Target: 5'- uGUUCAUUUUGGACGaaugcAAGCa- -3' miRNA: 3'- gCAGGUAAAGCCUGCaauauUUCGca -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 2975 | 0.66 | 0.999985 |
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Target: 5'- uCGUCCcgcuaauuUUUCGGGCGaguaAUAgucGAGCGUg -3' miRNA: 3'- -GCAGGu-------AAAGCCUGCaa--UAU---UUCGCA- -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 100772 | 0.68 | 0.999726 |
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Target: 5'- aGUCCGcUUCGGGCa----AAAGCGUa -3' miRNA: 3'- gCAGGUaAAGCCUGcaauaUUUCGCA- -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 26617 | 0.69 | 0.999444 |
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Target: 5'- aCGUUCAUUgucaugCGGGCGUgucgcGGAGCGc -3' miRNA: 3'- -GCAGGUAAa-----GCCUGCAaua--UUUCGCa -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 95346 | 0.7 | 0.998939 |
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Target: 5'- uCGUCCAcgcgCGGACGUUAgucacGucGCGg -3' miRNA: 3'- -GCAGGUaaa-GCCUGCAAUa----UuuCGCa -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 125637 | 0.74 | 0.979136 |
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Target: 5'- --gCCGUUUCGGACGUuugcgUGUAGuuGGCGg -3' miRNA: 3'- gcaGGUAAAGCCUGCA-----AUAUU--UCGCa -5' |
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| 8571 | 3' | -45.3 | NC_002169.1 | + | 113647 | 1.09 | 0.031139 |
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Target: 5'- aCGUCCAUUUCGGACGUUAUAAAGCGUc -3' miRNA: 3'- -GCAGGUAAAGCCUGCAAUAUUUCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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