Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8573 | 3' | -41.1 | NC_002169.1 | + | 13528 | 0.66 | 1 |
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Target: 5'- gUAUCucggUGUGUCGGUgGAUCGAc-- -3' miRNA: 3'- -GUAGuaa-ACGCAGUUAaCUAGCUuuu -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 45572 | 0.66 | 1 |
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Target: 5'- cCGUCAgcucgUUGCGUagc-UGAUCGGAc- -3' miRNA: 3'- -GUAGUa----AACGCAguuaACUAGCUUuu -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 116321 | 0.66 | 1 |
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Target: 5'- uGUCAaaaGCGUCAAaucaaaguUUGAUCGAc-- -3' miRNA: 3'- gUAGUaaaCGCAGUU--------AACUAGCUuuu -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 46290 | 0.69 | 0.999999 |
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Target: 5'- aCAUCGUccGCGcUCAcccacaucacgcaaaAUUGGUCGAAGAa -3' miRNA: 3'- -GUAGUAaaCGC-AGU---------------UAACUAGCUUUU- -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 71201 | 0.7 | 0.999997 |
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Target: 5'- aAUCAUUUGCGUCAAaUGAa------ -3' miRNA: 3'- gUAGUAAACGCAGUUaACUagcuuuu -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 58715 | 0.73 | 0.999711 |
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Target: 5'- gCGUCGUcgGCGccgauagUAGUUGAUCGAGAAa -3' miRNA: 3'- -GUAGUAaaCGCa------GUUAACUAGCUUUU- -5' |
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| 8573 | 3' | -41.1 | NC_002169.1 | + | 126816 | 1.08 | 0.086401 |
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Target: 5'- cCAUCAUUUGCGUCAAUUGAUCGAAAAu -3' miRNA: 3'- -GUAGUAAACGCAGUUAACUAGCUUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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