Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8573 | 5' | -54.4 | NC_002169.1 | + | 108436 | 0.7 | 0.722794 |
Target: 5'- gUGCUgUUCGCucgCGACGuGuACGCCAGCGAa -3' miRNA: 3'- -ACGA-AAGCG---GCUGU-C-UGCGGUCGUUc -5' |
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8573 | 5' | -54.4 | NC_002169.1 | + | 112262 | 0.72 | 0.660713 |
Target: 5'- aUGUUugacUUCGUCGA-GGACGCCGGCGAc -3' miRNA: 3'- -ACGA----AAGCGGCUgUCUGCGGUCGUUc -5' |
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8573 | 5' | -54.4 | NC_002169.1 | + | 119615 | 0.69 | 0.800591 |
Target: 5'- uUGCUUUCGUCGAagaucuuGGACGaaAGCAAa -3' miRNA: 3'- -ACGAAAGCGGCUg------UCUGCggUCGUUc -5' |
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8573 | 5' | -54.4 | NC_002169.1 | + | 120219 | 0.69 | 0.800591 |
Target: 5'- uUGCUUUCGUCGAagaucuuGGACGaaAGCAAa -3' miRNA: 3'- -ACGAAAGCGGCUg------UCUGCggUCGUUc -5' |
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8573 | 5' | -54.4 | NC_002169.1 | + | 120418 | 0.68 | 0.835948 |
Target: 5'- aUGUUggCGCUGAC-GACGCCcuuGCAAa -3' miRNA: 3'- -ACGAaaGCGGCUGuCUGCGGu--CGUUc -5' |
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8573 | 5' | -54.4 | NC_002169.1 | + | 126854 | 1.09 | 0.003003 |
Target: 5'- uUGCUUUCGCCGACAGACGCCAGCAAGa -3' miRNA: 3'- -ACGAAAGCGGCUGUCUGCGGUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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