Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8573 | 5' | -54.4 | NC_002169.1 | + | 62612 | 0.7 | 0.772357 |
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Target: 5'- cGUUUgUCGCUGACGagcgauGugGUCGGCAGGa -3' miRNA: 3'- aCGAA-AGCGGCUGU------CugCGGUCGUUC- -5' |
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| 8573 | 5' | -54.4 | NC_002169.1 | + | 108436 | 0.7 | 0.722794 |
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Target: 5'- gUGCUgUUCGCucgCGACGuGuACGCCAGCGAa -3' miRNA: 3'- -ACGA-AAGCG---GCUGU-C-UGCGGUCGUUc -5' |
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| 8573 | 5' | -54.4 | NC_002169.1 | + | 112262 | 0.72 | 0.660713 |
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Target: 5'- aUGUUugacUUCGUCGA-GGACGCCGGCGAc -3' miRNA: 3'- -ACGA----AAGCGGCUgUCUGCGGUCGUUc -5' |
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| 8573 | 5' | -54.4 | NC_002169.1 | + | 53136 | 0.73 | 0.576874 |
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Target: 5'- uUGCUUUCGUCGACgaucuuGGACGaaAGCAAa -3' miRNA: 3'- -ACGAAAGCGGCUG------UCUGCggUCGUUc -5' |
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| 8573 | 5' | -54.4 | NC_002169.1 | + | 11184 | 0.73 | 0.576874 |
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Target: 5'- uUGCUUUCGUCGACgaucuuGGACGaaAGCAAa -3' miRNA: 3'- -ACGAAAGCGGCUG------UCUGCggUCGUUc -5' |
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| 8573 | 5' | -54.4 | NC_002169.1 | + | 126854 | 1.09 | 0.003003 |
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Target: 5'- uUGCUUUCGCCGACAGACGCCAGCAAGa -3' miRNA: 3'- -ACGAAAGCGGCUGUCUGCGGUCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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