Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8575 | 3' | -49.6 | NC_002169.1 | + | 37108 | 0.66 | 0.997067 |
Target: 5'- uGCG-UGUCGgcGuuGAAGUCggccacguagccgaCCUCGCa -3' miRNA: 3'- gCGCaACAGCuuCu-CUUUAG--------------GGAGCG- -5' |
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8575 | 3' | -49.6 | NC_002169.1 | + | 13508 | 0.68 | 0.986125 |
Target: 5'- uGaCGUUGUCGAuGAGAAcgUgCaUCGCu -3' miRNA: 3'- gC-GCAACAGCUuCUCUUuaGgG-AGCG- -5' |
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8575 | 3' | -49.6 | NC_002169.1 | + | 22205 | 0.69 | 0.97226 |
Target: 5'- uCGCGUUccgcgucgcccacGUCGggGuuGGcUCCCUUGCc -3' miRNA: 3'- -GCGCAA-------------CAGCuuCucUUuAGGGAGCG- -5' |
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8575 | 3' | -49.6 | NC_002169.1 | + | 109552 | 0.7 | 0.962972 |
Target: 5'- -uCGaUGUCGAAGAGGAgaggcAUUgCUCGCa -3' miRNA: 3'- gcGCaACAGCUUCUCUU-----UAGgGAGCG- -5' |
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8575 | 3' | -49.6 | NC_002169.1 | + | 131299 | 1.15 | 0.005457 |
Target: 5'- aCGCGUUGUCGAAGAGAAAUCCCUCGCg -3' miRNA: 3'- -GCGCAACAGCUUCUCUUUAGGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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