Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8575 | 5' | -51.5 | NC_002169.1 | + | 26650 | 0.66 | 0.984506 |
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Target: 5'- aCGGCGUuGAUgUUCUuUUCGGCAAUg -3' miRNA: 3'- -GUCGCAuCUGaGAGGuAAGCCGUUGu -5' |
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| 8575 | 5' | -51.5 | NC_002169.1 | + | 99780 | 0.66 | 0.984506 |
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Target: 5'- uGGCGUAcaaUCUCC--UCGGCGGCc -3' miRNA: 3'- gUCGCAUcugAGAGGuaAGCCGUUGu -5' |
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| 8575 | 5' | -51.5 | NC_002169.1 | + | 74263 | 0.66 | 0.980391 |
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Target: 5'- aAGCGgaggUAGAguuucUUCUUCGUUgGGCAGCAu -3' miRNA: 3'- gUCGC----AUCU-----GAGAGGUAAgCCGUUGU- -5' |
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| 8575 | 5' | -51.5 | NC_002169.1 | + | 45990 | 0.66 | 0.975511 |
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Target: 5'- gAGCGUcuacggGGACUCggagaUCGGCGACGa -3' miRNA: 3'- gUCGCA------UCUGAGagguaAGCCGUUGU- -5' |
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| 8575 | 5' | -51.5 | NC_002169.1 | + | 132830 | 0.67 | 0.972476 |
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Target: 5'- aAGUGUAuGCUCgacaCCAccgaguaagugggUUCGGCGACAa -3' miRNA: 3'- gUCGCAUcUGAGa---GGU-------------AAGCCGUUGU- -5' |
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| 8575 | 5' | -51.5 | NC_002169.1 | + | 131335 | 0.94 | 0.05625 |
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Target: 5'- gCAGCGUAGACUCUCCAUUCGaCGACAc -3' miRNA: 3'- -GUCGCAUCUGAGAGGUAAGCcGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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