Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8576 | 3' | -56.1 | NC_002169.1 | + | 134203 | 1.08 | 0.002786 |
Target: 5'- aGACCGCUGCCGCCCGAAUUUUGCGCGc -3' miRNA: 3'- -CUGGCGACGGCGGGCUUAAAACGCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 26950 | 0.66 | 0.865996 |
Target: 5'- cGGCgGUguccGCCGCCCGAAUcUUG-GCc -3' miRNA: 3'- -CUGgCGa---CGGCGGGCUUAaAACgCGc -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 22362 | 0.66 | 0.858316 |
Target: 5'- cGGCCaGCacgUGCCGgCCGGAaagaUUGCGCu -3' miRNA: 3'- -CUGG-CG---ACGGCgGGCUUaa--AACGCGc -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 117664 | 0.67 | 0.850427 |
Target: 5'- aACCGCcGCCGCCgcagccaacaaGAAga-UGCGCGc -3' miRNA: 3'- cUGGCGaCGGCGGg----------CUUaaaACGCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 45943 | 0.69 | 0.722431 |
Target: 5'- cACCGCcGCCGCCaGAucgagaGCGCGa -3' miRNA: 3'- cUGGCGaCGGCGGgCUuaaaa-CGCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 80885 | 0.7 | 0.67162 |
Target: 5'- cGAUCGCcaacgaUGCaCGCCCGAcaaggUGCGCa -3' miRNA: 3'- -CUGGCG------ACG-GCGGGCUuaaa-ACGCGc -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 129103 | 0.73 | 0.488951 |
Target: 5'- -uCCGUgauaGCCGCUCGGAcauaUUUUGCGCGu -3' miRNA: 3'- cuGGCGa---CGGCGGGCUU----AAAACGCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 18247 | 0.74 | 0.451121 |
Target: 5'- cGGCCGCcGCCGuCCuCGAGgggcUUUGCGUGa -3' miRNA: 3'- -CUGGCGaCGGC-GG-GCUUa---AAACGCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 116599 | 0.66 | 0.887723 |
Target: 5'- uGCUGCUGCCGCCUccgcuGUUguuguuguugUUGCGUu -3' miRNA: 3'- cUGGCGACGGCGGGcu---UAA----------AACGCGc -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 118924 | 0.66 | 0.880705 |
Target: 5'- -gUCGUUGUCGUCgGGAUUUUgGCGCc -3' miRNA: 3'- cuGGCGACGGCGGgCUUAAAA-CGCGc -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 80205 | 0.66 | 0.872724 |
Target: 5'- aAUCGCUGCCGCCCGcgaaaagaucgucGAUgacgUUGUuuauuaaaGCGu -3' miRNA: 3'- cUGGCGACGGCGGGC-------------UUAa---AACG--------CGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 92995 | 0.66 | 0.858316 |
Target: 5'- cGCCGUUGCCggguaaaaagucgguGCCCGAGUUgcucaauaauuCGCGg -3' miRNA: 3'- cUGGCGACGG---------------CGGGCUUAAaac--------GCGC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 18141 | 0.68 | 0.799111 |
Target: 5'- aGCgGC-GCUGCCCGAAgggugcUUUGCGaCGg -3' miRNA: 3'- cUGgCGaCGGCGGGCUUa-----AAACGC-GC- -5' |
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8576 | 3' | -56.1 | NC_002169.1 | + | 62068 | 0.72 | 0.578781 |
Target: 5'- cGAUCugUGCCGUCCGAGccaUUUGCGCGa -3' miRNA: 3'- -CUGGcgACGGCGGGCUUa--AAACGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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