Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8576 | 5' | -50.7 | NC_002169.1 | + | 130002 | 0.71 | 0.910862 |
Target: 5'- cGCUGCUCAcaaucuUUCGAGAUCGaaGCGCGa -3' miRNA: 3'- -CGGCGGGUcu----AAGCUUUAGU--UGCGCc -5' |
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8576 | 5' | -50.7 | NC_002169.1 | + | 22168 | 0.71 | 0.904559 |
Target: 5'- uGCCGCCgGGAaacUCccAAUCGAUGUGGa -3' miRNA: 3'- -CGGCGGgUCUa--AGcuUUAGUUGCGCC- -5' |
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8576 | 5' | -50.7 | NC_002169.1 | + | 1951 | 0.71 | 0.904559 |
Target: 5'- cGCCGCCgAuGGUgUCGuugucAUCGACGUGGg -3' miRNA: 3'- -CGGCGGgU-CUA-AGCuu---UAGUUGCGCC- -5' |
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8576 | 5' | -50.7 | NC_002169.1 | + | 25699 | 0.72 | 0.869387 |
Target: 5'- aCCGCCgGGcgUCGccgcCAACGCGGc -3' miRNA: 3'- cGGCGGgUCuaAGCuuuaGUUGCGCC- -5' |
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8576 | 5' | -50.7 | NC_002169.1 | + | 22148 | 0.73 | 0.845531 |
Target: 5'- -aCGCCCuGAcUUCGGcAGUgAGCGCGGg -3' miRNA: 3'- cgGCGGGuCU-AAGCU-UUAgUUGCGCC- -5' |
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8576 | 5' | -50.7 | NC_002169.1 | + | 134243 | 1.14 | 0.004398 |
Target: 5'- cGCCGCCCAGAUUCGAAAUCAACGCGGa -3' miRNA: 3'- -CGGCGGGUCUAAGCUUUAGUUGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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