Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 858 | 3' | -57.1 | NC_000898.1 | + | 147025 | 0.66 | 0.86792 |
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Target: 5'- aACCUCCAgACGCAUCCuGCcguGUGGAa -3' miRNA: 3'- gUGGGGGUaUGUGUGGGuCGu--CGUCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 153033 | 0.66 | 0.835724 |
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Target: 5'- gGCCCgaGUGCA-GCCCAGCuccccggAGCAGAc -3' miRNA: 3'- gUGGGggUAUGUgUGGGUCG-------UCGUCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 5579 | 0.67 | 0.811047 |
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Target: 5'- uCGCCCuCCAccgGCuCGCCCuGCGGCcGAc -3' miRNA: 3'- -GUGGG-GGUa--UGuGUGGGuCGUCGuCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 158747 | 0.67 | 0.811047 |
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Target: 5'- uCGCCCuCCAccgGCuCGCCCuGCGGCcGAc -3' miRNA: 3'- -GUGGG-GGUa--UGuGUGGGuCGUCGuCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 91780 | 0.67 | 0.793223 |
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Target: 5'- aUugCCCCAUA-AC-CCCAGaGGCAGGg -3' miRNA: 3'- -GugGGGGUAUgUGuGGGUCgUCGUCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 7535 | 0.68 | 0.774818 |
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Target: 5'- aCACCCCacaCAUACACGCa-GGCGGaUAGAc -3' miRNA: 3'- -GUGGGG---GUAUGUGUGggUCGUC-GUCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 160703 | 0.68 | 0.774818 |
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Target: 5'- aCACCCCacaCAUACACGCa-GGCGGaUAGAc -3' miRNA: 3'- -GUGGGG---GUAUGUGUGggUCGUC-GUCU- -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 1901 | 0.71 | 0.554514 |
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Target: 5'- cCGCCCuCCccGCACAguucgUCCAGCAGCGGu -3' miRNA: 3'- -GUGGG-GGuaUGUGU-----GGGUCGUCGUCu -5' |
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| 858 | 3' | -57.1 | NC_000898.1 | + | 155069 | 0.71 | 0.554514 |
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Target: 5'- cCGCCCuCCccGCACAguucgUCCAGCAGCGGu -3' miRNA: 3'- -GUGGG-GGuaUGUGU-----GGGUCGUCGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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