Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 858 | 5' | -57.6 | NC_000898.1 | + | 1945 | 0.66 | 0.830423 |
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Target: 5'- gACAgACGUGCGGAG-AUGC-CGuGAg -3' miRNA: 3'- aUGUgUGCGCGCCUCgUGCGuGCuCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 155113 | 0.66 | 0.830423 |
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Target: 5'- gACAgACGUGCGGAG-AUGC-CGuGAg -3' miRNA: 3'- aUGUgUGCGCGCCUCgUGCGuGCuCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 8994 | 0.68 | 0.749606 |
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Target: 5'- gGCgACACGCGCGGAccacucggGC-CGguCGGGGa -3' miRNA: 3'- aUG-UGUGCGCGCCU--------CGuGCguGCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 6487 | 0.69 | 0.68076 |
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Target: 5'- aGgGCugGgGCGGGacaCGCGUACGAGAa -3' miRNA: 3'- aUgUGugCgCGCCUc--GUGCGUGCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 159655 | 0.69 | 0.68076 |
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Target: 5'- aGgGCugGgGCGGGacaCGCGUACGAGAa -3' miRNA: 3'- aUgUGugCgCGCCUc--GUGCGUGCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 155992 | 0.71 | 0.599854 |
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Target: 5'- cGgACACGCGCGGuGgACG-GCGAGGg -3' miRNA: 3'- aUgUGUGCGCGCCuCgUGCgUGCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 2824 | 0.71 | 0.599854 |
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Target: 5'- cGgACACGCGCGGuGgACG-GCGAGGg -3' miRNA: 3'- aUgUGUGCGCGCCuCgUGCgUGCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 8889 | 0.72 | 0.51069 |
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Target: 5'- gGCGCGCGCGC---GCGCGUugGGGGa -3' miRNA: 3'- aUGUGUGCGCGccuCGUGCGugCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 162057 | 0.72 | 0.51069 |
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Target: 5'- gGCGCGCGCGC---GCGCGUugGGGGa -3' miRNA: 3'- aUGUGUGCGCGccuCGUGCGugCUCU- -5' |
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| 858 | 5' | -57.6 | NC_000898.1 | + | 152916 | 0.75 | 0.376732 |
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Target: 5'- cUACA-ACGCGCGG-GUACGCGCGuGAu -3' miRNA: 3'- -AUGUgUGCGCGCCuCGUGCGUGCuCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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