Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
859 | 5' | -58 | NC_000898.1 | + | 116774 | 0.66 | 0.859935 |
Target: 5'- gGCCGcuaaGgUCUgaaUGCUGUUCGUUUUCCCUg -3' miRNA: 3'- -UGGC----UgAGG---ACGAUAGGCAGGAGGGA- -5' |
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859 | 5' | -58 | NC_000898.1 | + | 153162 | 0.67 | 0.775778 |
Target: 5'- uCCGGC-CCgagcGCaGUCCGaCCUCCCa -3' miRNA: 3'- uGGCUGaGGa---CGaUAGGCaGGAGGGa -5' |
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859 | 5' | -58 | NC_000898.1 | + | 5571 | 0.68 | 0.709023 |
Target: 5'- cACCGGCUcgcCCUGCgg-CCGacgucCCUCCCc -3' miRNA: 3'- -UGGCUGA---GGACGauaGGCa----GGAGGGa -5' |
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859 | 5' | -58 | NC_000898.1 | + | 158739 | 0.68 | 0.709023 |
Target: 5'- cACCGGCUcgcCCUGCgg-CCGacgucCCUCCCc -3' miRNA: 3'- -UGGCUGA---GGACGauaGGCa----GGAGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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