Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8617 | 3' | -49.8 | NC_002229.2 | + | 886 | 0.66 | 0.997885 |
Target: 5'- cGGUcGUGCGcgcggGGcCGCGCGUGUGggaGCGa -3' miRNA: 3'- -UCAaUACGUa----CU-GCGCGUACGCa--UGC- -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 137825 | 0.66 | 0.997885 |
Target: 5'- cGGUcGUGCGcgcggGGcCGCGCGUGUGggaGCGa -3' miRNA: 3'- -UCAaUACGUa----CU-GCGCGUACGCa--UGC- -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 136023 | 0.68 | 0.988676 |
Target: 5'- cGUcAUGCAUaACugGCGCAUGCGUgaACGc -3' miRNA: 3'- uCAaUACGUAcUG--CGCGUACGCA--UGC- -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 2689 | 0.68 | 0.988676 |
Target: 5'- cGUcAUGCAUaACugGCGCAUGCGUgaACGc -3' miRNA: 3'- uCAaUACGUAcUG--CGCGUACGCA--UGC- -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 39282 | 0.68 | 0.987133 |
Target: 5'- -----cGCAUGugGCGUGUG-GUACa -3' miRNA: 3'- ucaauaCGUACugCGCGUACgCAUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 136091 | 0.69 | 0.986133 |
Target: 5'- uGGUUAUcugcGCAUGccugguuuucgacacACGCGCAUGCGcagaUACa -3' miRNA: 3'- -UCAAUA----CGUAC---------------UGCGCGUACGC----AUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 2621 | 0.69 | 0.986133 |
Target: 5'- uGGUUAUcugcGCAUGccugguuuucgacacACGCGCAUGCGcagaUACa -3' miRNA: 3'- -UCAAUA----CGUAC---------------UGCGCGUACGC----AUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 2750 | 0.71 | 0.953895 |
Target: 5'- cGUUuUGUGUGuagaGgGCGCAUGCGUACu -3' miRNA: 3'- uCAAuACGUAC----UgCGCGUACGCAUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 135961 | 0.71 | 0.953895 |
Target: 5'- cGUUuUGUGUGuagaGgGCGCAUGCGUACu -3' miRNA: 3'- uCAAuACGUAC----UgCGCGUACGCAUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 2678 | 0.78 | 0.683737 |
Target: 5'- -uUUGUGUGUGGaggGCGCAUGCGUACu -3' miRNA: 3'- ucAAUACGUACUg--CGCGUACGCAUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 136033 | 0.78 | 0.683737 |
Target: 5'- -uUUGUGUGUGGaggGCGCAUGCGUACu -3' miRNA: 3'- ucAAUACGUACUg--CGCGUACGCAUGc -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 2876 | 1.11 | 0.009705 |
Target: 5'- cAGUUAUGCAUGACGCGCAUGCGUACGg -3' miRNA: 3'- -UCAAUACGUACUGCGCGUACGCAUGC- -5' |
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8617 | 3' | -49.8 | NC_002229.2 | + | 135835 | 1.11 | 0.009705 |
Target: 5'- cAGUUAUGCAUGACGCGCAUGCGUACGg -3' miRNA: 3'- -UCAAUACGUACUGCGCGUACGCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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