Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8618 | 3' | -58 | NC_002229.2 | + | 173700 | 0.66 | 0.810303 |
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Target: 5'- gUGUGGCCgcgagaGGgUUAgaGGCcGUGCGCa -3' miRNA: 3'- -ACACCGG------UCgAGUgaCCGaCACGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 138914 | 0.66 | 0.810303 |
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Target: 5'- gUGUGGCCgcgagaGGgUUAgaGGCcGUGCGCa -3' miRNA: 3'- -ACACCGG------UCgAGUgaCCGaCACGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 47593 | 0.66 | 0.810303 |
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Target: 5'- aUGUGGCCcuucGCgcgcaaUCAUUGGCUGUuGUAUUu -3' miRNA: 3'- -ACACCGGu---CG------AGUGACCGACA-CGUGA- -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 137707 | 0.67 | 0.774065 |
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Target: 5'- cGUGGCCcuggGGCUUg--GGCUGaGCGCa -3' miRNA: 3'- aCACCGG----UCGAGugaCCGACaCGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 1004 | 0.67 | 0.774065 |
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Target: 5'- cGUGGCCcuggGGCUUg--GGCUGaGCGCa -3' miRNA: 3'- aCACCGG----UCGAGugaCCGACaCGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 59564 | 0.67 | 0.764667 |
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Target: 5'- cGacGCCGGCaUCACUGGUgcgGUGUAUa -3' miRNA: 3'- aCacCGGUCG-AGUGACCGa--CACGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 112883 | 0.68 | 0.675905 |
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Target: 5'- cGUGGCCAa-UCAUUGGCUagaacguacaGUGCAUg -3' miRNA: 3'- aCACCGGUcgAGUGACCGA----------CACGUGa -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 3783 | 1.08 | 0.001999 |
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Target: 5'- cUGUGGCCAGCUCACUGGCUGUGCACUg -3' miRNA: 3'- -ACACCGGUCGAGUGACCGACACGUGA- -5' |
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| 8618 | 3' | -58 | NC_002229.2 | + | 134928 | 1.08 | 0.001999 |
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Target: 5'- cUGUGGCCAGCUCACUGGCUGUGCACUg -3' miRNA: 3'- -ACACCGGUCGAGUGACCGACACGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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