miRNA: 3'- cuuaaaUCAACAu------UGGGGGCUCCCUU- -5'
miRNA: 3'- cuuaaaUCAACAu------UGGGGGCUCCCUU- -5'
miRNA: 3'- -CUUAAAUCAACAUUGGGGGCUCCCUU- -5'
miRNA: 3'- -CUUAAAUCAACAUUGGGGGCUCCCUU- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8620 | 5' | -48.7 | NC_002229.2 | + | 170889 | 0.67 | 0.996766 |
|
Target: 5'- -----cGGUUGUuguucacAUUCCCGAGGGAAc -3' miRNA: 3'- cuuaaaUCAACAu------UGGGGGCUCCCUU- -5' |
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| 8620 | 5' | -48.7 | NC_002229.2 | + | 141725 | 0.67 | 0.996766 |
|
Target: 5'- -----cGGUUGUuguucacAUUCCCGAGGGAAc -3' miRNA: 3'- cuuaaaUCAACAu------UGGGGGCUCCCUU- -5' |
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| 8620 | 5' | -48.7 | NC_002229.2 | + | 131783 | 1.07 | 0.018248 |
|
Target: 5'- cGAAUUUAGUUGUAACCCCCGAGGGAAg -3' miRNA: 3'- -CUUAAAUCAACAUUGGGGGCUCCCUU- -5' |
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| 8620 | 5' | -48.7 | NC_002229.2 | + | 6928 | 1.07 | 0.018248 |
|
Target: 5'- cGAAUUUAGUUGUAACCCCCGAGGGAAg -3' miRNA: 3'- -CUUAAAUCAACAUUGGGGGCUCCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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