Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8622 | 5' | -55.9 | NC_002229.2 | + | 44293 | 0.67 | 0.892197 |
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Target: 5'- cCUCCCucuuGGAUUGCAUggUGUCCUAa -3' miRNA: 3'- aGAGGGc---CCUGGUGUAuuGCAGGGUg -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 28151 | 0.68 | 0.864061 |
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Target: 5'- gUCUCCCGaaaACCAUccguuccccaugAUAgacACGUCCCACa -3' miRNA: 3'- -AGAGGGCcc-UGGUG------------UAU---UGCAGGGUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 118314 | 0.68 | 0.856504 |
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Target: 5'- aCUCCCauguuaucuGaGGACCACAUAAgGUacucUCCGCg -3' miRNA: 3'- aGAGGG---------C-CCUGGUGUAUUgCA----GGGUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 148385 | 0.68 | 0.848749 |
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Target: 5'- aCUCCgGgGGACCcuuauCAaGGCGUCUCGCu -3' miRNA: 3'- aGAGGgC-CCUGGu----GUaUUGCAGGGUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 164229 | 0.68 | 0.848749 |
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Target: 5'- aCUCCgGgGGACCcuuauCAaGGCGUCUCGCu -3' miRNA: 3'- aGAGGgC-CCUGGu----GUaUUGCAGGGUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 121190 | 0.69 | 0.824361 |
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Target: 5'- cCUCCUGGuaaauCCAUAUuGCGUCCgCGCa -3' miRNA: 3'- aGAGGGCCcu---GGUGUAuUGCAGG-GUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 46218 | 0.69 | 0.807237 |
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Target: 5'- aCUUgUGGGACguUAUGugGUCCCGg -3' miRNA: 3'- aGAGgGCCCUGguGUAUugCAGGGUg -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 46253 | 0.71 | 0.713687 |
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Target: 5'- aUCUCCCGGGACCACAaaaaaaucaAAUGcugggugcaaUUCCAUa -3' miRNA: 3'- -AGAGGGCCCUGGUGUa--------UUGC----------AGGGUG- -5' |
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| 8622 | 5' | -55.9 | NC_002229.2 | + | 46421 | 1.11 | 0.002721 |
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Target: 5'- aUCUCCCGGGACCACAUAACGUCCCACa -3' miRNA: 3'- -AGAGGGCCCUGGUGUAUUGCAGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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