Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8623 | 3' | -49.9 | NC_002229.2 | + | 62220 | 0.66 | 0.997774 |
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Target: 5'- -aGCGuCCAgUUGUCUGAGgaaGAGUCu -3' miRNA: 3'- uaCGC-GGUaAGCAGACUUaggCUUAG- -5' |
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| 8623 | 3' | -49.9 | NC_002229.2 | + | 138509 | 0.67 | 0.990905 |
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Target: 5'- uGUGgGCCGgg-GUCUGcgggaucgGAUCCGGAUCc -3' miRNA: 3'- -UACgCGGUaagCAGAC--------UUAGGCUUAG- -5' |
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| 8623 | 3' | -49.9 | NC_002229.2 | + | 87179 | 0.69 | 0.980684 |
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Target: 5'- uUGCGCC--UCGUCaaauAUCCGAAUUu -3' miRNA: 3'- uACGCGGuaAGCAGacu-UAGGCUUAG- -5' |
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| 8623 | 3' | -49.9 | NC_002229.2 | + | 171814 | 0.75 | 0.807582 |
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Target: 5'- -aGUGCCAUUCuUCUGGAUCUGAu-- -3' miRNA: 3'- uaCGCGGUAAGcAGACUUAGGCUuag -5' |
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| 8623 | 3' | -49.9 | NC_002229.2 | + | 140800 | 0.75 | 0.807582 |
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Target: 5'- -aGUGCCAUUCuUCUGGAUCUGAu-- -3' miRNA: 3'- uaCGCGGUAAGcAGACUUAGGCUuag -5' |
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| 8623 | 3' | -49.9 | NC_002229.2 | + | 77392 | 1.09 | 0.010655 |
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Target: 5'- gAUGCGCCAUUCGUCUGAAUCCGAAUCa -3' miRNA: 3'- -UACGCGGUAAGCAGACUUAGGCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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