Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8626 | 3' | -44.9 | NC_002229.2 | + | 173706 | 0.67 | 0.999979 |
Target: 5'- uACAGUGugUGGCCGCGAgaggguuagaggccgUGCGCAg -3' miRNA: 3'- -UGUCAUcuGCUGGUGCU---------------AUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 166523 | 0.68 | 0.99992 |
Target: 5'- -gGGUGGACGGCCGaGAUggaaGCACc -3' miRNA: 3'- ugUCAUCUGCUGGUgCUA----UGUGu -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 165723 | 0.66 | 0.999994 |
Target: 5'- aACGGaAGuuGACagGCGAUACACAu -3' miRNA: 3'- -UGUCaUCugCUGg-UGCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 164658 | 0.71 | 0.997452 |
Target: 5'- uCGGUGGugGGgcCCGCGGUAuCGCAg -3' miRNA: 3'- uGUCAUCugCU--GGUGCUAU-GUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 151481 | 0.82 | 0.749036 |
Target: 5'- gGCAGUAGACGACCACGGa----- -3' miRNA: 3'- -UGUCAUCUGCUGGUGCUaugugu -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 147956 | 0.71 | 0.997452 |
Target: 5'- uCGGUGGugGGgcCCGCGGUAuCGCAg -3' miRNA: 3'- uGUCAUCugCU--GGUGCUAU-GUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 146891 | 0.66 | 0.999994 |
Target: 5'- aACGGaAGuuGACagGCGAUACACAu -3' miRNA: 3'- -UGUCaUCugCUGg-UGCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 146092 | 0.68 | 0.99992 |
Target: 5'- -gGGUGGACGGCCGaGAUggaaGCACc -3' miRNA: 3'- ugUCAUCUGCUGGUgCUA----UGUGu -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 138908 | 0.67 | 0.999979 |
Target: 5'- uACAGUGugUGGCCGCGAgaggguuagaggccgUGCGCAg -3' miRNA: 3'- -UGUCAUcuGCUGGUGCU---------------AUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 132226 | 1.06 | 0.048611 |
Target: 5'- aACAGUAGACGACCACGAUACACAc -3' miRNA: 3'- -UGUCAUCUGCUGGUGCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 97008 | 0.66 | 0.999992 |
Target: 5'- -aGGUucuGGGCGACUAC-AUGCACGa -3' miRNA: 3'- ugUCA---UCUGCUGGUGcUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 76670 | 0.66 | 0.999994 |
Target: 5'- -aGGUAGGCGauGCCuguugggAUGAUGCACGa -3' miRNA: 3'- ugUCAUCUGC--UGG-------UGCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 60485 | 0.69 | 0.999764 |
Target: 5'- gAUGGUAGACG-CUGcCGAUAUACAg -3' miRNA: 3'- -UGUCAUCUGCuGGU-GCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 58767 | 0.66 | 0.999994 |
Target: 5'- cCAGUGGAUgaggagcacagaGGCgACGAUAUGCAu -3' miRNA: 3'- uGUCAUCUG------------CUGgUGCUAUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 32023 | 0.68 | 0.999894 |
Target: 5'- cCAGUAcGGCGAUCcCGAaGCACAc -3' miRNA: 3'- uGUCAU-CUGCUGGuGCUaUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 22975 | 0.69 | 0.999764 |
Target: 5'- --uGUAGGCGACC-CGAcACAUAu -3' miRNA: 3'- uguCAUCUGCUGGuGCUaUGUGU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 13037 | 0.68 | 0.999894 |
Target: 5'- uCAGUAGACucCUAUGAUACAgAa -3' miRNA: 3'- uGUCAUCUGcuGGUGCUAUGUgU- -5' |
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8626 | 3' | -44.9 | NC_002229.2 | + | 6485 | 1.06 | 0.048611 |
Target: 5'- aACAGUAGACGACCACGAUACACAc -3' miRNA: 3'- -UGUCAUCUGCUGGUGCUAUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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