Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8627 | 5' | -45.4 | NC_002229.2 | + | 6237 | 1.11 | 0.026697 |
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Target: 5'- aCAUUUACAUUAUACGGAGGGCCUUGAu -3' miRNA: 3'- -GUAAAUGUAAUAUGCCUCCCGGAACU- -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 132308 | 0.81 | 0.784686 |
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Target: 5'- gGUUUGCAUaAUACGGAGGGUUcUGAu -3' miRNA: 3'- gUAAAUGUAaUAUGCCUCCCGGaACU- -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 165488 | 0.7 | 0.999308 |
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Target: 5'- --gUUGCAgccccccgaGUACGGGGGGCCg--- -3' miRNA: 3'- guaAAUGUaa-------UAUGCCUCCCGGaacu -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 51968 | 0.67 | 0.999982 |
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Target: 5'- ---aUGCAUcccauucauuCGGAGGGCgUUGAa -3' miRNA: 3'- guaaAUGUAauau------GCCUCCCGgAACU- -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 94954 | 0.66 | 0.999992 |
|
Target: 5'- -----uCAUgugACGGAGGGCCg--- -3' miRNA: 3'- guaaauGUAauaUGCCUCCCGGaacu -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 147127 | 0.7 | 0.999308 |
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Target: 5'- --gUUGCAgccccccgaGUACGGGGGGCCg--- -3' miRNA: 3'- guaAAUGUaa-------UAUGCCUCCCGGaacu -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 91652 | 0.7 | 0.998944 |
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Target: 5'- ----aACA-UAUACGGAGGaCCUUGGc -3' miRNA: 3'- guaaaUGUaAUAUGCCUCCcGGAACU- -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 6404 | 0.81 | 0.784686 |
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Target: 5'- gGUUUGCAUaAUACGGAGGGUUcUGAu -3' miRNA: 3'- gUAAAUGUAaUAUGCCUCCCGGaACU- -5' |
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| 8627 | 5' | -45.4 | NC_002229.2 | + | 132474 | 1.11 | 0.026697 |
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Target: 5'- aCAUUUACAUUAUACGGAGGGCCUUGAu -3' miRNA: 3'- -GUAAAUGUAAUAUGCCUCCCGGAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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