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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 8635 | 3' | -56.5 | NC_002229.2 | + | 117675 | 0.66 | 0.922234 |
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Target: 5'- uUCGGUCaaaaGUUCCUgcgUACACGgUGGCGCg -3' miRNA: 3'- -GGCUAG----CAGGGGa--GUGUGC-ACCGUGa -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 153375 | 0.66 | 0.910999 |
|
Target: 5'- aCCGAUUGcugcauUCCCCUUAguaGCGacGGCACg -3' miRNA: 3'- -GGCUAGC------AGGGGAGUg--UGCa-CCGUGa -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 98502 | 0.66 | 0.922234 |
|
Target: 5'- ---uUCGUCagCCCUCAU-CGUGGCAUg -3' miRNA: 3'- ggcuAGCAG--GGGAGUGuGCACCGUGa -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 138480 | 0.68 | 0.857297 |
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Target: 5'- gCGGgguUCCCCcggCACACGUGGCGg- -3' miRNA: 3'- gGCUagcAGGGGa--GUGUGCACCGUga -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 231 | 0.68 | 0.857297 |
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Target: 5'- gCGGgguUCCCCcggCACACGUGGCGg- -3' miRNA: 3'- gGCUagcAGGGGa--GUGUGCACCGUga -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 135623 | 1.1 | 0.003045 |
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Target: 5'- cCCGAUCGUCCCCUCACACGUGGCACUg -3' miRNA: 3'- -GGCUAGCAGGGGAGUGUGCACCGUGA- -5' |
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| 8635 | 3' | -56.5 | NC_002229.2 | + | 3088 | 1.1 | 0.003045 |
|
Target: 5'- cCCGAUCGUCCCCUCACACGUGGCACUg -3' miRNA: 3'- -GGCUAGCAGGGGAGUGUGCACCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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