Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8636 | 3' | -52.8 | NC_002229.2 | + | 67711 | 0.67 | 0.968677 |
Target: 5'- aCGUUCACcgCUUUUACuccUGCGGCAg- -3' miRNA: 3'- gGCAAGUGa-GAAAGUGc--GCGCCGUga -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 3457 | 0.68 | 0.96218 |
Target: 5'- cCCuUUUGCUUgUUCACuGUGCGGCAUUa -3' miRNA: 3'- -GGcAAGUGAGaAAGUG-CGCGCCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 135254 | 0.68 | 0.96218 |
Target: 5'- cCCuUUUGCUUgUUCACuGUGCGGCAUUa -3' miRNA: 3'- -GGcAAGUGAGaAAGUG-CGCGCCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 122928 | 0.69 | 0.942003 |
Target: 5'- aCGUUCACaaUCUcuaUUCGCGgGCGGguUg -3' miRNA: 3'- gGCAAGUG--AGA---AAGUGCgCGCCguGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 151982 | 0.69 | 0.927061 |
Target: 5'- cCCGaugUCGCUCggUUAUGUGCGGaUACa -3' miRNA: 3'- -GGCa--AGUGAGaaAGUGCGCGCC-GUGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 934 | 0.7 | 0.899936 |
Target: 5'- cCCGUUCGgUCUacucacagagcccCGCGCGCGGCuCa -3' miRNA: 3'- -GGCAAGUgAGAaa-----------GUGCGCGCCGuGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 137777 | 0.7 | 0.899936 |
Target: 5'- cCCGUUCGgUCUacucacagagcccCGCGCGCGGCuCa -3' miRNA: 3'- -GGCAAGUgAGAaa-----------GUGCGCGCCGuGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 29345 | 0.71 | 0.883801 |
Target: 5'- uCCGUcagCACUCUggcCACGCGUagaaGGUGCUg -3' miRNA: 3'- -GGCAa--GUGAGAaa-GUGCGCG----CCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 138039 | 0.72 | 0.812372 |
Target: 5'- aCGgcgUCGCUCccaCACGCGCGGCcccGCg -3' miRNA: 3'- gGCa--AGUGAGaaaGUGCGCGCCG---UGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 673 | 0.72 | 0.812372 |
Target: 5'- aCGgcgUCGCUCccaCACGCGCGGCcccGCg -3' miRNA: 3'- gGCa--AGUGAGaaaGUGCGCGCCG---UGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 129188 | 0.73 | 0.774126 |
Target: 5'- uCCGUUCGaagcagugauuaUCUUUCugGgGgGGCACUa -3' miRNA: 3'- -GGCAAGUg-----------AGAAAGugCgCgCCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 9524 | 0.74 | 0.727688 |
Target: 5'- uCCGUUCgaagcagugauuAUUCUUUCugGgGgGGCACUa -3' miRNA: 3'- -GGCAAG------------UGAGAAAGugCgCgCCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 135498 | 0.92 | 0.089497 |
Target: 5'- uCCGUUCGCUCUUUCAUGCGCaGCAUUa -3' miRNA: 3'- -GGCAAGUGAGAAAGUGCGCGcCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 3213 | 0.92 | 0.089497 |
Target: 5'- uCCGUUCGCUCUUUCAUGCGCaGCAUUa -3' miRNA: 3'- -GGCAAGUGAGAAAGUGCGCGcCGUGA- -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 2970 | 1.06 | 0.011756 |
Target: 5'- cCCGUUCACUCUUUCACGCGCGGCAUa -3' miRNA: 3'- -GGCAAGUGAGAAAGUGCGCGCCGUGa -5' |
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8636 | 3' | -52.8 | NC_002229.2 | + | 135742 | 1.11 | 0.005697 |
Target: 5'- cCCGUUCACUCUUUCACGCGCGGCACUa -3' miRNA: 3'- -GGCAAGUGAGAAAGUGCGCGCCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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