Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
864 | 3' | -55.3 | NC_000898.1 | + | 3041 | 0.67 | 0.892522 |
Target: 5'- ---gGGCGGCGCggaCGAgggcGCCGGCGgGa -3' miRNA: 3'- gauaUCGUCGCGg--GCUa---CGGUUGCgC- -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 156208 | 0.67 | 0.892522 |
Target: 5'- ---gGGCGGCGCggaCGAgggcGCCGGCGgGa -3' miRNA: 3'- gauaUCGUCGCGg--GCUa---CGGUUGCgC- -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 8347 | 0.68 | 0.863881 |
Target: 5'- ---cGGCAcgucGCGCCCGAUGCCGu---- -3' miRNA: 3'- gauaUCGU----CGCGGGCUACGGUugcgc -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 161514 | 0.68 | 0.863881 |
Target: 5'- ---cGGCAcgucGCGCCCGAUGCCGu---- -3' miRNA: 3'- gauaUCGU----CGCGGGCUACGGUugcgc -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 3116 | 0.7 | 0.73032 |
Target: 5'- ---gGGCGGcCGCgaccugCCGAUGCaCGGCGCGg -3' miRNA: 3'- gauaUCGUC-GCG------GGCUACG-GUUGCGC- -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 156283 | 0.7 | 0.73032 |
Target: 5'- ---gGGCGGcCGCgaccugCCGAUGCaCGGCGCGg -3' miRNA: 3'- gauaUCGUC-GCG------GGCUACG-GUUGCGC- -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 2371 | 0.72 | 0.659503 |
Target: 5'- -cGUGGCGGCGCgCGccGUCAuCGCGg -3' miRNA: 3'- gaUAUCGUCGCGgGCuaCGGUuGCGC- -5' |
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864 | 3' | -55.3 | NC_000898.1 | + | 155538 | 0.72 | 0.659503 |
Target: 5'- -cGUGGCGGCGCgCGccGUCAuCGCGg -3' miRNA: 3'- gaUAUCGUCGCGgGCuaCGGUuGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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