Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8640 | 5' | -60.6 | NC_002229.2 | + | 138577 | 0.84 | 0.054095 |
Target: 5'- aUAGCUACG-GCAnACAGCCCGGCCAn -3' miRNA: 3'- -AUCGAUGCgCGUcUGUCGGGCCGGUu -5' |
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8640 | 5' | -60.6 | NC_002229.2 | + | 134 | 0.84 | 0.054095 |
Target: 5'- aUAGCUACG-GCAnACAGCCCGGCCAn -3' miRNA: 3'- -AUCGAUGCgCGUcUGUCGGGCCGGUu -5' |
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8640 | 5' | -60.6 | NC_002229.2 | + | 138552 | 1.04 | 0.001993 |
Target: 5'- aUAGCUACGCGCAGACAGCCCGGCCAAu -3' miRNA: 3'- -AUCGAUGCGCGUCUGUCGGGCCGGUU- -5' |
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8640 | 5' | -60.6 | NC_002229.2 | + | 159 | 1.04 | 0.001993 |
Target: 5'- aUAGCUACGCGCAGACAGCCCGGCCAAu -3' miRNA: 3'- -AUCGAUGCGCGUCUGUCGGGCCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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