Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8647 | 3' | -58 | NC_002229.2 | + | 138672 | 0.66 | 0.87697 |
Target: 5'- gGGGCaCgUGCaUGGGGCGUgGCGgGAGAu -3' miRNA: 3'- -CCUG-GgACG-GCCUUGCGgUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 39 | 0.66 | 0.87697 |
Target: 5'- gGGGCaCgUGCaUGGGGCGUgGCGgGAGAu -3' miRNA: 3'- -CCUG-GgACG-GCCUUGCGgUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 57734 | 0.66 | 0.86992 |
Target: 5'- aGGAgCUggUGUCGGAAuCGCgGCGCGAa- -3' miRNA: 3'- -CCUgGG--ACGGCCUU-GCGgUGUGCUcu -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 126199 | 0.66 | 0.86267 |
Target: 5'- aGGACggcgagaaaUGCgGGGACGCCAgAUGAGc -3' miRNA: 3'- -CCUGgg-------ACGgCCUUGCGGUgUGCUCu -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 968 | 0.66 | 0.86267 |
Target: 5'- gGGACCggGCUcGAGCGCCcaccucCGCGGGGg -3' miRNA: 3'- -CCUGGgaCGGcCUUGCGGu-----GUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 137743 | 0.66 | 0.86267 |
Target: 5'- gGGACCggGCUcGAGCGCCcaccucCGCGGGGg -3' miRNA: 3'- -CCUGGgaCGGcCUUGCGGu-----GUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 76663 | 0.67 | 0.839773 |
Target: 5'- cGAUgCCUGuuGGGAUGauGCACGAGAc -3' miRNA: 3'- cCUG-GGACggCCUUGCggUGUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 135090 | 0.67 | 0.815284 |
Target: 5'- aGGCCUgauUGCUGGGACGCgG-AUGAGAg -3' miRNA: 3'- cCUGGG---ACGGCCUUGCGgUgUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 3622 | 0.67 | 0.815284 |
Target: 5'- aGGCCUgauUGCUGGGACGCgG-AUGAGAg -3' miRNA: 3'- cCUGGG---ACGGCCUUGCGgUgUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 144798 | 0.67 | 0.815284 |
Target: 5'- cGGCUCgGCCGGAcuugggaggACGCC-CACGuGGg -3' miRNA: 3'- cCUGGGaCGGCCU---------UGCGGuGUGCuCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 167816 | 0.67 | 0.815284 |
Target: 5'- cGGCUCgGCCGGAcuugggaggACGCC-CACGuGGg -3' miRNA: 3'- cCUGGGaCGGCCU---------UGCGGuGUGCuCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 36984 | 0.67 | 0.815284 |
Target: 5'- cGGAUuuUagCGGAAUGCCgaACACGAGAa -3' miRNA: 3'- -CCUGggAcgGCCUUGCGG--UGUGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 1050 | 0.68 | 0.780484 |
Target: 5'- aGGACUCggcGCCuGGGCGCCgGCACGGc- -3' miRNA: 3'- -CCUGGGa--CGGcCUUGCGG-UGUGCUcu -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 137662 | 0.68 | 0.780484 |
Target: 5'- aGGACUCggcGCCuGGGCGCCgGCACGGc- -3' miRNA: 3'- -CCUGGGa--CGGcCUUGCGG-UGUGCUcu -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 138352 | 0.7 | 0.676095 |
Target: 5'- gGGAgCC-GCCccagGGGGCGCCGCGgGGGGg -3' miRNA: 3'- -CCUgGGaCGG----CCUUGCGGUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 359 | 0.7 | 0.676095 |
Target: 5'- gGGAgCC-GCCccagGGGGCGCCGCGgGGGGg -3' miRNA: 3'- -CCUgGGaCGG----CCUUGCGGUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 164650 | 0.71 | 0.63357 |
Target: 5'- gGGGCCCgcgguaucgcagacUGCuCGGGugGCUGCGgGAGGg -3' miRNA: 3'- -CCUGGG--------------ACG-GCCUugCGGUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 147964 | 0.71 | 0.63357 |
Target: 5'- gGGGCCCgcgguaucgcagacUGCuCGGGugGCUGCGgGAGGg -3' miRNA: 3'- -CCUGGG--------------ACG-GCCUugCGGUGUgCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 137908 | 0.71 | 0.58708 |
Target: 5'- uGGugCCcGCUGG-GCGCCGCuCGGGGc -3' miRNA: 3'- -CCugGGaCGGCCuUGCGGUGuGCUCU- -5' |
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8647 | 3' | -58 | NC_002229.2 | + | 804 | 0.71 | 0.58708 |
Target: 5'- uGGugCCcGCUGG-GCGCCGCuCGGGGc -3' miRNA: 3'- -CCugGGaCGGCCuUGCGGUGuGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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