Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8648 | 3' | -57.1 | NC_002229.2 | + | 148837 | 0.66 | 0.879903 |
Target: 5'- uACCGCAcuuugcaauuGGgGGGgGggGGGGGGGGAuCGa -3' miRNA: 3'- -UGGCGU----------UUgUCCgCa-CCCUCUCCU-GC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 163777 | 0.66 | 0.879903 |
Target: 5'- uACCGCAcuuugcaauuGGgGGGgGggGGGGGGGGAuCGa -3' miRNA: 3'- -UGGCGU----------UUgUCCgCa-CCCUCUCCU-GC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 5732 | 0.66 | 0.872835 |
Target: 5'- -aCGgAGACGGGgG-GGGGGGGGAa- -3' miRNA: 3'- ugGCgUUUGUCCgCaCCCUCUCCUgc -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 132979 | 0.66 | 0.872835 |
Target: 5'- -aCGgAGACGGGgG-GGGGGGGGAa- -3' miRNA: 3'- ugGCgUUUGUCCgCaCCCUCUCCUgc -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 142343 | 0.67 | 0.86556 |
Target: 5'- -aCGCaAAACAGGUGUucgccuGGGAGGGG-UGa -3' miRNA: 3'- ugGCG-UUUGUCCGCA------CCCUCUCCuGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 145964 | 0.67 | 0.86556 |
Target: 5'- gGCCGCAAugccuaAGGCGgucGGGAauaguAGGACa -3' miRNA: 3'- -UGGCGUUug----UCCGCa--CCCUc----UCCUGc -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 170271 | 0.67 | 0.86556 |
Target: 5'- -aCGCaAAACAGGUGUucgccuGGGAGGGG-UGa -3' miRNA: 3'- ugGCG-UUUGUCCGCA------CCCUCUCCuGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 166650 | 0.67 | 0.86556 |
Target: 5'- gGCCGCAAugccuaAGGCGgucGGGAauaguAGGACa -3' miRNA: 3'- -UGGCGUUug----UCCGCa--CCCUc----UCCUGc -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 688 | 0.68 | 0.800573 |
Target: 5'- gACCGCGGccuGCGGGCGaggaccccaGGGcggauGGGGGCGa -3' miRNA: 3'- -UGGCGUU---UGUCCGCa--------CCCu----CUCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 138023 | 0.68 | 0.800573 |
Target: 5'- gACCGCGGccuGCGGGCGaggaccccaGGGcggauGGGGGCGa -3' miRNA: 3'- -UGGCGUU---UGUCCGCa--------CCCu----CUCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 831 | 0.69 | 0.773566 |
Target: 5'- -gCGCAGGgGGGCGcGGGGGuugccccgGGGGCGc -3' miRNA: 3'- ugGCGUUUgUCCGCaCCCUC--------UCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 137880 | 0.69 | 0.773566 |
Target: 5'- -gCGCAGGgGGGCGcGGGGGuugccccgGGGGCGc -3' miRNA: 3'- ugGCGUUUgUCCGCaCCCUC--------UCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 164639 | 0.69 | 0.745461 |
Target: 5'- uAUCGCAGACugcucGGGUGgcugcGGGAGGGGcaGCGg -3' miRNA: 3'- -UGGCGUUUG-----UCCGCa----CCCUCUCC--UGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 147975 | 0.69 | 0.745461 |
Target: 5'- uAUCGCAGACugcucGGGUGgcugcGGGAGGGGcaGCGg -3' miRNA: 3'- -UGGCGUUUG-----UCCGCa----CCCUCUCC--UGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 105743 | 0.69 | 0.72623 |
Target: 5'- cGCCGUGAgccacaACGGGCGaauugacguaGGGaAGAGGGCGg -3' miRNA: 3'- -UGGCGUU------UGUCCGCa---------CCC-UCUCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 138334 | 0.69 | 0.72623 |
Target: 5'- cGCCGCGGGgGGGa--GGGGGuGGGGCGg -3' miRNA: 3'- -UGGCGUUUgUCCgcaCCCUC-UCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 377 | 0.69 | 0.72623 |
Target: 5'- cGCCGCGGGgGGGa--GGGGGuGGGGCGg -3' miRNA: 3'- -UGGCGUUUgUCCgcaCCCUC-UCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 150915 | 0.71 | 0.656906 |
Target: 5'- cUCGCAuAUGGGgGUGGGAcacAGGACGa -3' miRNA: 3'- uGGCGUuUGUCCgCACCCUc--UCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 145033 | 0.95 | 0.021771 |
Target: 5'- aACCGCAAACAGGCGUGGaGGAGGACGc -3' miRNA: 3'- -UGGCGUUUGUCCGCACCcUCUCCUGC- -5' |
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8648 | 3' | -57.1 | NC_002229.2 | + | 167582 | 0.95 | 0.021771 |
Target: 5'- aACCGCAAACAGGCGUGGaGGAGGACGc -3' miRNA: 3'- -UGGCGUUUGUCCGCACCcUCUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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