miRNA: 3'- -CUGGUGUAUCCUCGCGCCGGUGAUGU- -5'
miRNA: 3'- -CUGGUGUAUCCUCGCGCCGGUGAUGU- -5'
miRNA: 3'- cUGGugUAUCCUCG-----CGCCG-GUGAUGu -5'
miRNA: 3'- -CUGG---UGUAUCCUCGcGCCGGUGaUGu -5'
miRNA: 3'- cUGgUGuAUCCUCGCGCCgGUGAUGu -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8653 | 5' | -56.1 | NC_002229.2 | + | 168731 | 1.08 | 0.00348 |
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Target: 5'- gGACCACAUAGGAGCGCGGCCACUACAc -3' miRNA: 3'- -CUGGUGUAUCCUCGCGCCGGUGAUGU- -5' |
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| 8653 | 5' | -56.1 | NC_002229.2 | + | 143883 | 1.08 | 0.00348 |
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Target: 5'- gGACCACAUAGGAGCGCGGCCACUACAc -3' miRNA: 3'- -CUGGUGUAUCCUCGCGCCGGUGAUGU- -5' |
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| 8653 | 5' | -56.1 | NC_002229.2 | + | 67635 | 0.7 | 0.740235 |
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Target: 5'- uAUCugGUGGGGGUuaugaGCGGCgCACUGCu -3' miRNA: 3'- cUGGugUAUCCUCG-----CGCCG-GUGAUGu -5' |
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| 8653 | 5' | -56.1 | NC_002229.2 | + | 17453 | 0.69 | 0.778373 |
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Target: 5'- aGGCCuggGCGUAGGAGUaCGGCUACaACu -3' miRNA: 3'- -CUGG---UGUAUCCUCGcGCCGGUGaUGu -5' |
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| 8653 | 5' | -56.1 | NC_002229.2 | + | 79727 | 0.66 | 0.910961 |
|
Target: 5'- cGCgGCuUGGGGGCGUGGaUACUACu -3' miRNA: 3'- cUGgUGuAUCCUCGCGCCgGUGAUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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