Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8656 | 5' | -49.9 | NC_002229.2 | + | 172791 | 0.97 | 0.060468 |
Target: 5'- cCGAAGCAUGGAAACGUCCUGG-GAAAa -3' miRNA: 3'- -GCUUCGUACCUUUGCAGGACCuCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 166787 | 0.72 | 0.911933 |
Target: 5'- uGGAG-AUGGAAGCGagUCUGGAGAAc -3' miRNA: 3'- gCUUCgUACCUUUGCa-GGACCUCUUu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 166022 | 0.69 | 0.979106 |
Target: 5'- cCGAgAGuCAUGaGAACGUCCUGGGGu-- -3' miRNA: 3'- -GCU-UC-GUACcUUUGCAGGACCUCuuu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 165432 | 0.72 | 0.911933 |
Target: 5'- gGAAugcGCAggcGGAGACGgagCCUGGGGAGGg -3' miRNA: 3'- gCUU---CGUa--CCUUUGCa--GGACCUCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 165306 | 0.74 | 0.828773 |
Target: 5'- gGggGCAggugaggGGAGGCGUgCUGGGGGc- -3' miRNA: 3'- gCuuCGUa------CCUUUGCAgGACCUCUuu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 147308 | 0.74 | 0.828773 |
Target: 5'- gGggGCAggugaggGGAGGCGUgCUGGGGGc- -3' miRNA: 3'- gCuuCGUa------CCUUUGCAgGACCUCUuu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 147182 | 0.72 | 0.911933 |
Target: 5'- gGAAugcGCAggcGGAGACGgagCCUGGGGAGGg -3' miRNA: 3'- gCUU---CGUa--CCUUUGCa--GGACCUCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 146592 | 0.69 | 0.979106 |
Target: 5'- cCGAgAGuCAUGaGAACGUCCUGGGGu-- -3' miRNA: 3'- -GCU-UC-GUACcUUUGCAGGACCUCuuu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 145827 | 0.72 | 0.911933 |
Target: 5'- uGGAG-AUGGAAGCGagUCUGGAGAAc -3' miRNA: 3'- gCUUCgUACCUUUGCa-GGACCUCUUu -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 139823 | 0.97 | 0.060468 |
Target: 5'- cCGAAGCAUGGAAACGUCCUGG-GAAAa -3' miRNA: 3'- -GCUUCGUACCUUUGCAGGACCuCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 128338 | 0.74 | 0.845938 |
Target: 5'- gGAAGCGcGGGGACGUUgaUGGAGGAGu -3' miRNA: 3'- gCUUCGUaCCUUUGCAGg-ACCUCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 69185 | 0.74 | 0.845938 |
Target: 5'- aGAAGCGUGGAucaaaGUCUgcggGGAGGAAu -3' miRNA: 3'- gCUUCGUACCUuug--CAGGa---CCUCUUU- -5' |
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8656 | 5' | -49.9 | NC_002229.2 | + | 10374 | 0.74 | 0.845938 |
Target: 5'- gGAAGCGcGGGGACGUUgaUGGAGGAGu -3' miRNA: 3'- gCUUCGUaCCUUUGCAGg-ACCUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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