miRNA: 3'- aaaAAGUGguUU-UUGCGCGCGGUgac -5'
miRNA: 3'- aaaAAGUGguUU-UUGCGCGCGGUgac -5'
miRNA: 3'- aaaaAGuGGU--UUUUGCGCGCG-GUGac -5'
miRNA: 3'- aaaaAGuGGU--UUUUGCGCGCG-GUGac -5'
miRNA: 3'- aaAAAGUGGUUUUU-GCG----CGCGGUGAc -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 866 | 5' | -48.2 | NC_000898.1 | + | 2376 | 0.66 | 0.999174 |
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Target: 5'- --aUUCGCguGGcGGCGCGCGCCGu-- -3' miRNA: 3'- aaaAAGUGguUU-UUGCGCGCGGUgac -5' |
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| 866 | 5' | -48.2 | NC_000898.1 | + | 155543 | 0.66 | 0.999174 |
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Target: 5'- --aUUCGCguGGcGGCGCGCGCCGu-- -3' miRNA: 3'- aaaAAGUGguUU-UUGCGCGCGGUgac -5' |
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| 866 | 5' | -48.2 | NC_000898.1 | + | 8899 | 0.68 | 0.99486 |
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Target: 5'- ---cUC-CCAuaGAGGCGCGCGCgCGCg- -3' miRNA: 3'- aaaaAGuGGU--UUUUGCGCGCG-GUGac -5' |
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| 866 | 5' | -48.2 | NC_000898.1 | + | 162067 | 0.68 | 0.99486 |
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Target: 5'- ---cUC-CCAuaGAGGCGCGCGCgCGCg- -3' miRNA: 3'- aaaaAGuGGU--UUUUGCGCGCG-GUGac -5' |
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| 866 | 5' | -48.2 | NC_000898.1 | + | 38020 | 0.69 | 0.990709 |
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Target: 5'- -aUUUUAUCGAAAAuCGCcaggGCGCCACUu -3' miRNA: 3'- aaAAAGUGGUUUUU-GCG----CGCGGUGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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