Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8664 | 3' | -48.9 | NC_002229.2 | + | 135495 | 1.12 | 0.009063 |
Target: 5'- gUUCGCUCUUUCAUGCGCAGCAUUACCg -3' miRNA: 3'- -AAGCGAGAAAGUACGCGUCGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 3216 | 1.12 | 0.009063 |
Target: 5'- gUUCGCUCUUUCAUGCGCAGCAUUACCg -3' miRNA: 3'- -AAGCGAGAAAGUACGCGUCGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 135739 | 0.76 | 0.79641 |
Target: 5'- gUUCaCUCUUUCAcGCGCGGCAcUAUCg -3' miRNA: 3'- -AAGcGAGAAAGUaCGCGUCGUaAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 2973 | 0.74 | 0.882103 |
Target: 5'- gUUCaCUCUUUCAcGCGCGGCA-UAUCg -3' miRNA: 3'- -AAGcGAGAAAGUaCGCGUCGUaAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 3454 | 0.73 | 0.909805 |
Target: 5'- uUUUGCUUgUUCAcUGUGCGGCAUUAUUa -3' miRNA: 3'- -AAGCGAGaAAGU-ACGCGUCGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 135257 | 0.73 | 0.909805 |
Target: 5'- uUUUGCUUgUUCAcUGUGCGGCAUUAUUa -3' miRNA: 3'- -AAGCGAGaAAGU-ACGCGUCGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 2164 | 0.72 | 0.933275 |
Target: 5'- uUUCGuCUCUggCAUGUGCAGCAa---- -3' miRNA: 3'- -AAGC-GAGAaaGUACGCGUCGUaaugg -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 136548 | 0.72 | 0.933275 |
Target: 5'- uUUCGuCUCUggCAUGUGCAGCAa---- -3' miRNA: 3'- -AAGC-GAGAaaGUACGCGUCGUaaugg -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 894 | 0.68 | 0.991282 |
Target: 5'- --gGCUCcaacggUCGUGCGC-GCGggGCCg -3' miRNA: 3'- aagCGAGaa----AGUACGCGuCGUaaUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 137817 | 0.68 | 0.991282 |
Target: 5'- --gGCUCcaacggUCGUGCGC-GCGggGCCg -3' miRNA: 3'- aagCGAGaa----AGUACGCGuCGUaaUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 151988 | 0.68 | 0.99438 |
Target: 5'- gUCGCUCggUUAUGUGCGGa--UACa -3' miRNA: 3'- aAGCGAGaaAGUACGCGUCguaAUGg -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 142715 | 0.67 | 0.995898 |
Target: 5'- aUCGCUC----GUGuCGCAaauGCGUUACCu -3' miRNA: 3'- aAGCGAGaaagUAC-GCGU---CGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 169899 | 0.67 | 0.995898 |
Target: 5'- aUCGCUC----GUGuCGCAaauGCGUUACCu -3' miRNA: 3'- aAGCGAGaaagUAC-GCGU---CGUAAUGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 125164 | 0.67 | 0.996521 |
Target: 5'- aUCGagaCUUUCGUGCuGCAGCugu-CCa -3' miRNA: 3'- aAGCga-GAAAGUACG-CGUCGuaauGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 11613 | 0.66 | 0.999051 |
Target: 5'- aUUCaGCUCgUUCGUcgccuaGCGUAGCGUU-CCu -3' miRNA: 3'- -AAG-CGAGaAAGUA------CGCGUCGUAAuGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 127100 | 0.66 | 0.999051 |
Target: 5'- aUUCaGCUCgUUCGUcgccuaGCGUAGCGUU-CCu -3' miRNA: 3'- -AAG-CGAGaAAGUA------CGCGUCGUAAuGG- -5' |
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8664 | 3' | -48.9 | NC_002229.2 | + | 100062 | 0.66 | 0.998585 |
Target: 5'- aUgGCUUcUUCAUGCGUuGCAUUGa- -3' miRNA: 3'- aAgCGAGaAAGUACGCGuCGUAAUgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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