Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 8671 | 3' | -47 | NC_002229.2 | + | 132509 | 1.06 | 0.029548 |
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Target: 5'- gGAUCAGAACCCUCCGUAUUAUGCAAAc -3' miRNA: 3'- -CUAGUCUUGGGAGGCAUAAUACGUUU- -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 6202 | 1.06 | 0.029548 |
|
Target: 5'- gGAUCAGAACCCUCCGUAUUAUGCAAAc -3' miRNA: 3'- -CUAGUCUUGGGAGGCAUAAUACGUUU- -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 132272 | 0.81 | 0.642696 |
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Target: 5'- cGAUCAaGGCCCUCCGUAUaAUGUAAAu -3' miRNA: 3'- -CUAGUcUUGGGAGGCAUAaUACGUUU- -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 6439 | 0.8 | 0.718979 |
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Target: 5'- cGAUCAaGGCCCUCCGUAUaAUGUAAu -3' miRNA: 3'- -CUAGUcUUGGGAGGCAUAaUACGUUu -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 118822 | 0.68 | 0.999091 |
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Target: 5'- gGAUCAGAACaUCUCC-UcgUAUGCGc- -3' miRNA: 3'- -CUAGUCUUG-GGAGGcAuaAUACGUuu -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 5949 | 0.68 | 0.998882 |
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Target: 5'- uAUCGGAACCCUUCGUucgGUGa--- -3' miRNA: 3'- cUAGUCUUGGGAGGCAuaaUACguuu -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 146265 | 0.68 | 0.99834 |
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Target: 5'- -cUCAGGGCCUgCCGUGUuuuccggcaUGUGCAc- -3' miRNA: 3'- cuAGUCUUGGGaGGCAUA---------AUACGUuu -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 166349 | 0.68 | 0.99834 |
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Target: 5'- -cUCAGGGCCUgCCGUGUuuuccggcaUGUGCAc- -3' miRNA: 3'- cuAGUCUUGGGaGGCAUA---------AUACGUuu -5' |
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| 8671 | 3' | -47 | NC_002229.2 | + | 132762 | 0.68 | 0.998882 |
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Target: 5'- uAUCGGAACCCUUCGUucgGUGa--- -3' miRNA: 3'- cUAGUCUUGGGAGGCAuaaUACguuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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