Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8674 | 5' | -46.6 | NC_002229.2 | + | 754 | 0.66 | 0.999965 |
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Target: 5'- aUCUCCCGGGccCCGCcGAGGcuGA-CAGAg -3' miRNA: 3'- -AGAGGGCCCu-GGUGuUUUU--UUaGUUU- -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 137957 | 0.66 | 0.999965 |
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Target: 5'- aUCUCCCGGGccCCGCcGAGGcuGA-CAGAg -3' miRNA: 3'- -AGAGGGCCCu-GGUGuUUUU--UUaGUUU- -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 79321 | 0.68 | 0.99951 |
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Target: 5'- cUUUCUCGGGAUgGCGAGAGGgauauAUCAu- -3' miRNA: 3'- -AGAGGGCCCUGgUGUUUUUU-----UAGUuu -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 42527 | 0.69 | 0.998592 |
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Target: 5'- cUCUCCCcgaauuaaacuGGGCgAUAAAGAAAUCAAAa -3' miRNA: 3'- -AGAGGGc----------CCUGgUGUUUUUUUAGUUU- -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 91405 | 0.69 | 0.998592 |
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Target: 5'- aCUgCCGugguuagaGGGCCACAGGAAAgcAUCAAAa -3' miRNA: 3'- aGAgGGC--------CCUGGUGUUUUUU--UAGUUU- -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 46421 | 0.82 | 0.66605 |
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Target: 5'- aUCUCCCGGGACCACAuaac-GUCc-- -3' miRNA: 3'- -AGAGGGCCCUGGUGUuuuuuUAGuuu -5' |
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| 8674 | 5' | -46.6 | NC_002229.2 | + | 46253 | 1.08 | 0.028422 |
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Target: 5'- aUCUCCCGGGACCACAAAAAAAUCAAAu -3' miRNA: 3'- -AGAGGGCCCUGGUGUUUUUUUAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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