Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8677 | 5' | -48.7 | NC_002229.2 | + | 86981 | 0.66 | 0.99944 |
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Target: 5'- --aAUUCGGAUauuuGACGA--GGCGCa -3' miRNA: 3'- uacUAAGCCUAagu-CUGCUuaCCGCG- -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 153105 | 0.67 | 0.998725 |
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Target: 5'- ----gUCGGGUcugUCgGGugGAAUGGCGa -3' miRNA: 3'- uacuaAGCCUA---AG-UCugCUUACCGCg -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 143941 | 0.67 | 0.998725 |
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Target: 5'- aAUGAauuaUCGGAUcUCAGAgcagcUGuuuAUGGCGCg -3' miRNA: 3'- -UACUa---AGCCUA-AGUCU-----GCu--UACCGCG- -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 168673 | 0.67 | 0.998725 |
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Target: 5'- aAUGAauuaUCGGAUcUCAGAgcagcUGuuuAUGGCGCg -3' miRNA: 3'- -UACUa---AGCCUA-AGUCU-----GCu--UACCGCG- -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 77353 | 0.67 | 0.998457 |
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Target: 5'- -aGAUUCcGAUUCGGACGAuu--CGCa -3' miRNA: 3'- uaCUAAGcCUAAGUCUGCUuaccGCG- -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 66979 | 0.67 | 0.998142 |
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Target: 5'- --aAUUCGGAUacCAGAUGcGUGGgGCu -3' miRNA: 3'- uacUAAGCCUAa-GUCUGCuUACCgCG- -5' |
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| 8677 | 5' | -48.7 | NC_002229.2 | + | 77190 | 1.12 | 0.010968 |
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Target: 5'- aAUGAUUCGGAUUCAGACGAAUGGCGCa -3' miRNA: 3'- -UACUAAGCCUAAGUCUGCUUACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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