Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8678 | 3' | -46.5 | NC_002229.2 | + | 92753 | 0.66 | 0.999991 |
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Target: 5'- -aGGGAGUaaAGACGuUCAUCAaaUcgGCa -3' miRNA: 3'- cgUCCUUAgcUCUGC-AGUAGU--AuaCG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 168631 | 0.67 | 0.999942 |
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Target: 5'- cGCAGcAGUCGGGACGUgugCAUCcc--GCa -3' miRNA: 3'- -CGUCcUUAGCUCUGCA---GUAGuauaCG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 143983 | 0.67 | 0.999942 |
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Target: 5'- cGCAGcAGUCGGGACGUgugCAUCcc--GCa -3' miRNA: 3'- -CGUCcUUAGCUCUGCA---GUAGuauaCG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 77321 | 0.67 | 0.999923 |
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Target: 5'- uGUAGGggUCuGAuGAUgcguGUCAUCAuguuucgggUAUGCg -3' miRNA: 3'- -CGUCCuuAG-CU-CUG----CAGUAGU---------AUACG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 121827 | 0.67 | 0.999923 |
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Target: 5'- -aGGGAAUCGuaugaagggGGugGgucaauuguaUCAUCGUAUGUg -3' miRNA: 3'- cgUCCUUAGC---------UCugC----------AGUAGUAUACG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 160335 | 0.67 | 0.999899 |
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Target: 5'- uGguGGAuaucgCGGGAuCGUCGUUGUA-GCa -3' miRNA: 3'- -CguCCUua---GCUCU-GCAGUAGUAUaCG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 139824 | 0.7 | 0.99846 |
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Target: 5'- uCGGGAAUCGAGGaaUGUCGUCcaauuaaagaagAUcAUGCa -3' miRNA: 3'- cGUCCUUAGCUCU--GCAGUAG------------UA-UACG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 172790 | 0.7 | 0.99846 |
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Target: 5'- uCGGGAAUCGAGGaaUGUCGUCcaauuaaagaagAUcAUGCa -3' miRNA: 3'- cGUCCUUAGCUCU--GCAGUAG------------UA-UACG- -5' |
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| 8678 | 3' | -46.5 | NC_002229.2 | + | 110261 | 1.17 | 0.01001 |
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Target: 5'- aGCAGGAAUCGAGACGUCAUCAUAUGCa -3' miRNA: 3'- -CGUCCUUAGCUCUGCAGUAGUAUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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