Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8678 | 5' | -53.3 | NC_002229.2 | + | 81203 | 0.66 | 0.987059 |
Target: 5'- aGGGGAAUGuCGgCGaUCCGAUguugCUGc -3' miRNA: 3'- -UCCCUUGCuGCgGCaAGGCUAaa--GGC- -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 12274 | 0.67 | 0.97482 |
Target: 5'- cGGGGACGAgacguUGCCGUU-CGA--UCCa -3' miRNA: 3'- uCCCUUGCU-----GCGGCAAgGCUaaAGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 126439 | 0.67 | 0.97482 |
Target: 5'- cGGGGACGAgacguUGCCGUU-CGA--UCCa -3' miRNA: 3'- uCCCUUGCU-----GCGGCAAgGCUaaAGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 174072 | 0.67 | 0.962952 |
Target: 5'- cGGGGGCugcgaGAUGCCGggCUGAgcUUUCCc -3' miRNA: 3'- uCCCUUG-----CUGCGGCaaGGCU--AAAGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 138543 | 0.67 | 0.962952 |
Target: 5'- cGGGGGCugcgaGAUGCCGggCUGAgcUUUCCc -3' miRNA: 3'- uCCCUUG-----CUGCGGCaaGGCU--AAAGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 138095 | 0.68 | 0.955752 |
Target: 5'- cGGGAGCGGCGCCc--CCGGggcaacccCCGc -3' miRNA: 3'- uCCCUUGCUGCGGcaaGGCUaaa-----GGC- -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 617 | 0.68 | 0.955752 |
Target: 5'- cGGGAGCGGCGCCc--CCGGggcaacccCCGc -3' miRNA: 3'- uCCCUUGCUGCGGcaaGGCUaaa-----GGC- -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 110492 | 0.72 | 0.78986 |
Target: 5'- -cGGAACGGCGUCGUUCCcuugGAUaUCCc -3' miRNA: 3'- ucCCUUGCUGCGGCAAGG----CUAaAGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 860 | 0.77 | 0.531946 |
Target: 5'- uGGGAGCGACGCCGU-CCGcgcgCCc -3' miRNA: 3'- uCCCUUGCUGCGGCAaGGCuaaaGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 137851 | 0.77 | 0.531946 |
Target: 5'- uGGGAGCGACGCCGU-CCGcgcgCCc -3' miRNA: 3'- uCCCUUGCUGCGGCAaGGCuaaaGGc -5' |
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8678 | 5' | -53.3 | NC_002229.2 | + | 110300 | 1.08 | 0.007829 |
Target: 5'- aAGGGAACGACGCCGUUCCGAUUUCCGc -3' miRNA: 3'- -UCCCUUGCUGCGGCAAGGCUAAAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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