Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8681 | 3' | -55.7 | NC_002229.2 | + | 3466 | 0.66 | 0.91779 |
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Target: 5'- aCUCuCUUCCCCUUuugcuuGUUCACUGUGc -3' miRNA: 3'- -GAG-GAAGGGGGGccu---CAAGUGACAUa -5' |
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| 8681 | 3' | -55.7 | NC_002229.2 | + | 135245 | 0.66 | 0.91779 |
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Target: 5'- aCUCuCUUCCCCUUuugcuuGUUCACUGUGc -3' miRNA: 3'- -GAG-GAAGGGGGGccu---CAAGUGACAUa -5' |
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| 8681 | 3' | -55.7 | NC_002229.2 | + | 131987 | 0.67 | 0.872084 |
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Target: 5'- uUCCUUCCCUCgGGGGUUacaACUa--- -3' miRNA: 3'- gAGGAAGGGGGgCCUCAAg--UGAcaua -5' |
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| 8681 | 3' | -55.7 | NC_002229.2 | + | 6725 | 0.67 | 0.872084 |
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Target: 5'- uUCCUUCCCUCgGGGGUUacaACUa--- -3' miRNA: 3'- gAGGAAGGGGGgCCUCAAg--UGAcaua -5' |
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| 8681 | 3' | -55.7 | NC_002229.2 | + | 6963 | 1.05 | 0.00612 |
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Target: 5'- uCUCCUUCCCCCCGGAGUUCACUGUAUc -3' miRNA: 3'- -GAGGAAGGGGGGCCUCAAGUGACAUA- -5' |
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| 8681 | 3' | -55.7 | NC_002229.2 | + | 131748 | 1.05 | 0.00612 |
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Target: 5'- uCUCCUUCCCCCCGGAGUUCACUGUAUc -3' miRNA: 3'- -GAGGAAGGGGGGCCUCAAGUGACAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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