Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8687 | 3' | -68.6 | NC_002229.2 | + | 165697 | 0.66 | 0.430601 |
Target: 5'- aCCGaaagcCGGC-CCCCCguacucggGGGGCuGCaacuUCCCg -3' miRNA: 3'- -GGC-----GCCGcGGGGGa-------CCCCGcCG----AGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 146917 | 0.66 | 0.430601 |
Target: 5'- aCCGaaagcCGGC-CCCCCguacucggGGGGCuGCaacuUCCCg -3' miRNA: 3'- -GGC-----GCCGcGGGGGa-------CCCCGcCG----AGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 147126 | 0.66 | 0.414657 |
Target: 5'- uUGCaGC-CCCCCgaguacggGGGGCcGGCUUUCg -3' miRNA: 3'- gGCGcCGcGGGGGa-------CCCCG-CCGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 165489 | 0.66 | 0.414657 |
Target: 5'- uUGCaGC-CCCCCgaguacggGGGGCcGGCUUUCg -3' miRNA: 3'- gGCGcCGcGGGGGa-------CCCCG-CCGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 12635 | 0.66 | 0.406823 |
Target: 5'- gCGCGGCccuccGCCccuuuuccaCCCUGGGGagcgggGGCgaCCCa -3' miRNA: 3'- gGCGCCG-----CGG---------GGGACCCCg-----CCGa-GGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 126077 | 0.66 | 0.406823 |
Target: 5'- gCGCGGCccuccGCCccuuuuccaCCCUGGGGagcgggGGCgaCCCa -3' miRNA: 3'- gGCGCCG-----CGG---------GGGACCCCg-----CCGa-GGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 137910 | 0.68 | 0.320402 |
Target: 5'- -gGUGGUGCCCgCUGGGcGCcGCUCg- -3' miRNA: 3'- ggCGCCGCGGGgGACCC-CGcCGAGgg -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 802 | 0.68 | 0.320402 |
Target: 5'- -gGUGGUGCCCgCUGGGcGCcGCUCg- -3' miRNA: 3'- ggCGCCGCGGGgGACCC-CGcCGAGgg -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 138068 | 0.69 | 0.282039 |
Target: 5'- ----cGCGCCCCCcugcgccgUGGGGCGcgcggacggcgucGCUCCCa -3' miRNA: 3'- ggcgcCGCGGGGG--------ACCCCGC-------------CGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 644 | 0.69 | 0.282039 |
Target: 5'- ----cGCGCCCCCcugcgccgUGGGGCGcgcggacggcgucGCUCCCa -3' miRNA: 3'- ggcgcCGCGGGGG--------ACCCCGC-------------CGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 138091 | 0.75 | 0.111752 |
Target: 5'- -aGCGGCGCCCCC-GGGGCaaCcCCCg -3' miRNA: 3'- ggCGCCGCGGGGGaCCCCGccGaGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 621 | 0.75 | 0.111752 |
Target: 5'- -aGCGGCGCCCCC-GGGGCaaCcCCCg -3' miRNA: 3'- ggCGCCGCGGGGGaCCCCGccGaGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 821 | 0.76 | 0.094603 |
Target: 5'- gCGCGGggguUGCCCCg-GGGGCGccGCUCCCg -3' miRNA: 3'- gGCGCC----GCGGGGgaCCCCGC--CGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 137890 | 0.76 | 0.094603 |
Target: 5'- gCGCGGggguUGCCCCg-GGGGCGccGCUCCCg -3' miRNA: 3'- gGCGCC----GCGGGGgaCCCCGC--CGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 558 | 1.1 | 0.000263 |
Target: 5'- cCCGCGGCGCCCCCUGGGGCGGCUCCCc -3' miRNA: 3'- -GGCGCCGCGGGGGACCCCGCCGAGGG- -5' |
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8687 | 3' | -68.6 | NC_002229.2 | + | 138153 | 1.1 | 0.000263 |
Target: 5'- cCCGCGGCGCCCCCUGGGGCGGCUCCCc -3' miRNA: 3'- -GGCGCCGCGGGGGACCCCGCCGAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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