miRNA: 3'- gACUU-----UCaGUCGCUCAUUUGUC--GCCC- -5'
miRNA: 3'- -GACUUUCAGUCGCUCAUUUGUCGCCC- -5'
miRNA: 3'- -GACUUUCAGUCGCUCAUUUGUCGCCC- -5'
miRNA: 3'- gACUU-----UCaGUCGCUCAUUUGUC--GCCC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8687 | 5' | -48.7 | NC_002229.2 | + | 6525 | 0.67 | 0.998534 |
|
Target: 5'- uUGAGauaugGGaCGGUGGGUGAGCAGgaUGGGa -3' miRNA: 3'- gACUU-----UCaGUCGCUCAUUUGUC--GCCC- -5' |
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| 8687 | 5' | -48.7 | NC_002229.2 | + | 524 | 1.11 | 0.013315 |
|
Target: 5'- gCUGAAAGUCAGCGAGUAAACAGCGGGc -3' miRNA: 3'- -GACUUUCAGUCGCUCAUUUGUCGCCC- -5' |
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| 8687 | 5' | -48.7 | NC_002229.2 | + | 138187 | 1.11 | 0.013315 |
|
Target: 5'- gCUGAAAGUCAGCGAGUAAACAGCGGGc -3' miRNA: 3'- -GACUUUCAGUCGCUCAUUUGUCGCCC- -5' |
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| 8687 | 5' | -48.7 | NC_002229.2 | + | 132186 | 0.67 | 0.998534 |
|
Target: 5'- uUGAGauaugGGaCGGUGGGUGAGCAGgaUGGGa -3' miRNA: 3'- gACUU-----UCaGUCGCUCAUUUGUC--GCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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