Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
869 | 5' | -58.7 | NC_000898.1 | + | 10788 | 0.68 | 0.689562 |
Target: 5'- uUCC-CUGCGGGACGuGaucUGCGGUGCGa -3' miRNA: 3'- cAGGaGGCGCUCUGC-C---AUGUCGCGCa -5' |
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869 | 5' | -58.7 | NC_000898.1 | + | 2714 | 0.67 | 0.73812 |
Target: 5'- uUCCUCCGCGGGACcugGCGG-GCa- -3' miRNA: 3'- cAGGAGGCGCUCUGccaUGUCgCGca -5' |
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869 | 5' | -58.7 | NC_000898.1 | + | 155882 | 0.67 | 0.73812 |
Target: 5'- uUCCUCCGCGGGACcugGCGG-GCa- -3' miRNA: 3'- cAGGAGGCGCUCUGccaUGUCgCGca -5' |
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869 | 5' | -58.7 | NC_000898.1 | + | 13229 | 0.66 | 0.819273 |
Target: 5'- uGUCUguuUCUGCuGAGACGcGUcaaACAGCGCa- -3' miRNA: 3'- -CAGG---AGGCG-CUCUGC-CA---UGUCGCGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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