Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8690 | 5' | -45.6 | NC_002229.2 | + | 17062 | 0.71 | 0.998096 |
Target: 5'- aGCGACaaGAGGUCUuacacgugCUACGGGAaugUUACg -3' miRNA: 3'- -CGUUGagCUCUAGA--------GAUGCUCU---AAUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 48057 | 0.67 | 0.999977 |
Target: 5'- uCGAgUCGAgGGUCUCguuuuaauggACGAGGUUAUu -3' miRNA: 3'- cGUUgAGCU-CUAGAGa---------UGCUCUAAUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 108878 | 0.67 | 0.999968 |
Target: 5'- aGCGACUCGAaGcAUCUCaaAUGGGAcgACa -3' miRNA: 3'- -CGUUGAGCU-C-UAGAGa-UGCUCUaaUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 124098 | 0.66 | 0.999996 |
Target: 5'- aCAGcCUCGAGAUUUCUACcaaaguGAGuccUACg -3' miRNA: 3'- cGUU-GAGCUCUAGAGAUG------CUCua-AUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 140061 | 1.15 | 0.016205 |
Target: 5'- aGCAACUCGAGAUCUCUACGAGAUUACa -3' miRNA: 3'- -CGUUGAGCUCUAGAGAUGCUCUAAUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 140184 | 0.92 | 0.29141 |
Target: 5'- gGCAACUCGAaAUCUCUACGAGAUaACa -3' miRNA: 3'- -CGUUGAGCUcUAGAGAUGCUCUAaUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 140223 | 0.67 | 0.999977 |
Target: 5'- ----aUCGGGAUCUCccCGAGAUaACa -3' miRNA: 3'- cguugAGCUCUAGAGauGCUCUAaUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 147978 | 0.68 | 0.999868 |
Target: 5'- cGCAgacuGCUCGGGugg-CUGCGGGAgggGCa -3' miRNA: 3'- -CGU----UGAGCUCuagaGAUGCUCUaa-UG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 164636 | 0.68 | 0.999868 |
Target: 5'- cGCAgacuGCUCGGGugg-CUGCGGGAgggGCa -3' miRNA: 3'- -CGU----UGAGCUCuagaGAUGCUCUaa-UG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 172391 | 0.67 | 0.999977 |
Target: 5'- ----aUCGGGAUCUCccCGAGAUaACa -3' miRNA: 3'- cguugAGCUCUAGAGauGCUCUAaUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 172430 | 0.92 | 0.29141 |
Target: 5'- gGCAACUCGAaAUCUCUACGAGAUaACa -3' miRNA: 3'- -CGUUGAGCUcUAGAGAUGCUCUAaUG- -5' |
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8690 | 5' | -45.6 | NC_002229.2 | + | 172553 | 1.15 | 0.016205 |
Target: 5'- aGCAACUCGAGAUCUCUACGAGAUUACa -3' miRNA: 3'- -CGUUGAGCUCUAGAGAUGCUCUAAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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