Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8694 | 3' | -55.1 | NC_002229.2 | + | 146209 | 0.66 | 0.939406 |
Target: 5'- --cUGGAuGAGGAUUGGuAUGggauUCCCGCu -3' miRNA: 3'- gucAUCUcCUCCUGACC-UAC----AGGGCG- -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 166405 | 0.66 | 0.939406 |
Target: 5'- --cUGGAuGAGGAUUGGuAUGggauUCCCGCu -3' miRNA: 3'- gucAUCUcCUCCUGACC-UAC----AGGGCG- -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 111355 | 0.69 | 0.826442 |
Target: 5'- aGGUGGAGGugggauaugggcAGGaACUGGAUGccgagcgCUCGCa -3' miRNA: 3'- gUCAUCUCC------------UCC-UGACCUACa------GGGCG- -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 145020 | 0.7 | 0.781436 |
Target: 5'- -cGUGGAGGAGGACgcccacgUGGgcGUCCUc- -3' miRNA: 3'- guCAUCUCCUCCUG-------ACCuaCAGGGcg -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 167595 | 0.7 | 0.781436 |
Target: 5'- -cGUGGAGGAGGACgcccacgUGGgcGUCCUc- -3' miRNA: 3'- guCAUCUCCUCCUG-------ACCuaCAGGGcg -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 61713 | 0.73 | 0.634593 |
Target: 5'- aUAGUAGAGGAaa--UGGAUG-CCCGCu -3' miRNA: 3'- -GUCAUCUCCUccugACCUACaGGGCG- -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 164876 | 1.11 | 0.003264 |
Target: 5'- cCAGUAGAGGAGGACUGGAUGUCCCGCc -3' miRNA: 3'- -GUCAUCUCCUCCUGACCUACAGGGCG- -5' |
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8694 | 3' | -55.1 | NC_002229.2 | + | 147738 | 1.11 | 0.003264 |
Target: 5'- cCAGUAGAGGAGGACUGGAUGUCCCGCc -3' miRNA: 3'- -GUCAUCUCCUCCUGACCUACAGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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