Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8696 | 3' | -60.8 | NC_002229.2 | + | 164455 | 1.07 | 0.001536 |
Target: 5'- cAGUCUGCGAUACCGCGGGCCCCACCAc -3' miRNA: 3'- -UCAGACGCUAUGGCGCCCGGGGUGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 737 | 0.72 | 0.393722 |
Target: 5'- gGGcUCUGUGAguagACCGaaCGGGCCCC-CCGc -3' miRNA: 3'- -UC-AGACGCUa---UGGC--GCCCGGGGuGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 137939 | 0.72 | 0.399437 |
Target: 5'- ----cGCGggGCCGCgcuccgcaucucccGGGCCCCGCCGa -3' miRNA: 3'- ucagaCGCuaUGGCG--------------CCCGGGGUGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 137229 | 0.69 | 0.545703 |
Target: 5'- uGUCUGUcg-GCCGCgagagguuuuaGGGCCCCGCgGg -3' miRNA: 3'- uCAGACGcuaUGGCG-----------CCCGGGGUGgU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 159693 | 0.67 | 0.643471 |
Target: 5'- uGGUCUGUGAaaggUGCCGCucuCCCCAUCGu -3' miRNA: 3'- -UCAGACGCU----AUGGCGcccGGGGUGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 170454 | 0.67 | 0.692455 |
Target: 5'- cAGUgCUGUGu--CCGCuGGCCCgGCCu -3' miRNA: 3'- -UCA-GACGCuauGGCGcCCGGGgUGGu -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 126290 | 0.66 | 0.711778 |
Target: 5'- -aUCUGUGAUGCCGagaGGGUCaaACUAu -3' miRNA: 3'- ucAGACGCUAUGGCg--CCCGGggUGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 1482 | 0.69 | 0.545703 |
Target: 5'- uGUCUGUcg-GCCGCgagagguuuuaGGGCCCCGCgGg -3' miRNA: 3'- uCAGACGcuaUGGCG-----------CCCGGGGUGgU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 772 | 0.72 | 0.399437 |
Target: 5'- ----cGCGggGCCGCgcuccgcaucucccGGGCCCCGCCGa -3' miRNA: 3'- ucagaCGCuaUGGCG--------------CCCGGGGUGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 137975 | 0.72 | 0.393722 |
Target: 5'- gGGcUCUGUGAguagACCGaaCGGGCCCC-CCGc -3' miRNA: 3'- -UC-AGACGCUa---UGGC--GCCCGGGGuGGU- -5' |
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8696 | 3' | -60.8 | NC_002229.2 | + | 148159 | 1.07 | 0.001536 |
Target: 5'- cAGUCUGCGAUACCGCGGGCCCCACCAc -3' miRNA: 3'- -UCAGACGCUAUGGCGCCCGGGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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