Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8698 | 5' | -55.3 | NC_002229.2 | + | 143438 | 1.01 | 0.012645 |
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Target: 5'- cGGCCCAGUACAGCCUGCGGCAaauGGGc -3' miRNA: 3'- -CCGGGUCAUGUCGGACGCCGUau-UCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 169176 | 1.01 | 0.012645 |
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Target: 5'- cGGCCCAGUACAGCCUGCGGCAaauGGGc -3' miRNA: 3'- -CCGGGUCAUGUCGGACGCCGUau-UCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 693 | 0.72 | 0.669548 |
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Target: 5'- cGGCCgAccGCGGCCUGCGGgc-GAGGa -3' miRNA: 3'- -CCGGgUcaUGUCGGACGCCguaUUCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 138018 | 0.72 | 0.669548 |
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Target: 5'- cGGCCgAccGCGGCCUGCGGgc-GAGGa -3' miRNA: 3'- -CCGGgUcaUGUCGGACGCCguaUUCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 2155 | 0.71 | 0.699823 |
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Target: 5'- uGGCau-GUGCAGCaaugGUGGUAUAAGGa -3' miRNA: 3'- -CCGgguCAUGUCGga--CGCCGUAUUCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 136557 | 0.71 | 0.699823 |
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Target: 5'- uGGCau-GUGCAGCaaugGUGGUAUAAGGa -3' miRNA: 3'- -CCGgguCAUGUCGga--CGCCGUAUUCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 17441 | 0.68 | 0.854624 |
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Target: 5'- cGGCCacgAGUuaGGCCUG-GGCGUAGGa -3' miRNA: 3'- -CCGGg--UCAugUCGGACgCCGUAUUCc -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 167640 | 0.67 | 0.903838 |
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Target: 5'- cGGCUCGaagaauCAGCCcGCGGUcUGGGGg -3' miRNA: 3'- -CCGGGUcau---GUCGGaCGCCGuAUUCC- -5' |
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| 8698 | 5' | -55.3 | NC_002229.2 | + | 144974 | 0.67 | 0.903838 |
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Target: 5'- cGGCUCGaagaauCAGCCcGCGGUcUGGGGg -3' miRNA: 3'- -CCGGGUcau---GUCGGaCGCCGuAUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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