Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 87 | 3' | -57.1 | AC_000006.1 | + | 33152 | 0.66 | 0.459077 |
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Target: 5'- gUCCAGaauguaGCCCCUGCUcaguuggGUGuuCUGGcUCc -3' miRNA: 3'- -GGGUCg-----CGGGGACGA-------UACu-GACCuAG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 19603 | 0.67 | 0.429978 |
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Target: 5'- gCCCGGCgGCCCgUGCgu---CUGGcgCg -3' miRNA: 3'- -GGGUCG-CGGGgACGauacuGACCuaG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 24375 | 0.68 | 0.364456 |
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Target: 5'- gCUAGCGCCggcgCCUGCUAcGGCcGcGGUCg -3' miRNA: 3'- gGGUCGCGG----GGACGAUaCUGaC-CUAG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 6575 | 0.68 | 0.347047 |
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Target: 5'- -aCAGCGCCCCccGCggAUG-CUGGcgCg -3' miRNA: 3'- ggGUCGCGGGGa-CGa-UACuGACCuaG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 768 | 0.7 | 0.269087 |
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Target: 5'- aCCCAGUGgguaCCCUGCU---GCUGGAa- -3' miRNA: 3'- -GGGUCGCg---GGGACGAuacUGACCUag -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 26150 | 0.74 | 0.139214 |
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Target: 5'- aUCAGCgagauGCCCCUGCgcc-GCUGGAUCa -3' miRNA: 3'- gGGUCG-----CGGGGACGauacUGACCUAG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 18784 | 0.76 | 0.101389 |
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Target: 5'- gCCCGGCGCCaCCUGCU-UGAUggGGcgCa -3' miRNA: 3'- -GGGUCGCGG-GGACGAuACUGa-CCuaG- -5' |
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| 87 | 3' | -57.1 | AC_000006.1 | + | 30973 | 1.12 | 0.000209 |
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Target: 5'- gCCCAGCGCCCCUGCUAUGACUGGAUCa -3' miRNA: 3'- -GGGUCGCGGGGACGAUACUGACCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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