Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
87 | 5' | -59.4 | AC_000006.1 | + | 20852 | 0.73 | 0.119571 |
Target: 5'- uACaGCUUC-GAGUACGAGGCCAUGCu -3' miRNA: 3'- -UGcUGGGGuCUCGUGCUCCGGUACGu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 7599 | 0.71 | 0.172208 |
Target: 5'- aGCGAUCCCAGcguaAGCGCGcGGCUAgaucGCGa -3' miRNA: 3'- -UGCUGGGGUC----UCGUGCuCCGGUa---CGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 7409 | 0.68 | 0.244583 |
Target: 5'- -aGGuCCUCGGGGCAUugcAGGCCGUGCu -3' miRNA: 3'- ugCU-GGGGUCUCGUGc--UCCGGUACGu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 23616 | 0.68 | 0.269365 |
Target: 5'- cGCGACCCCcaggcccuGGAGCAgCGGcgcaagcucaugcuGGCCGUGg- -3' miRNA: 3'- -UGCUGGGG--------UCUCGU-GCU--------------CCGGUACgu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 22676 | 0.67 | 0.324117 |
Target: 5'- gUGGCCCCGGGGCGgaGuGGCCucucGCu -3' miRNA: 3'- uGCUGGGGUCUCGUg-CuCCGGua--CGu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 8347 | 0.66 | 0.332232 |
Target: 5'- cCGGCCCCGcGGGCA-GGGGCg--GCAg -3' miRNA: 3'- uGCUGGGGU-CUCGUgCUCCGguaCGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 23706 | 0.66 | 0.332232 |
Target: 5'- aGCGACCCCgAGAccuGCGCaAGGUCGaggagaccUGCAc -3' miRNA: 3'- -UGCUGGGG-UCU---CGUGcUCCGGU--------ACGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 9682 | 0.66 | 0.340496 |
Target: 5'- gACG-CCCCuGAgcggcuGCACGAGcGCCAggucgGCGa -3' miRNA: 3'- -UGCuGGGGuCU------CGUGCUC-CGGUa----CGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 7480 | 0.66 | 0.340496 |
Target: 5'- -gGAagCCCAGAGCuCGcGGGCCAUGa- -3' miRNA: 3'- ugCUg-GGGUCUCGuGC-UCCGGUACgu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 5373 | 0.67 | 0.31615 |
Target: 5'- -aGGCCCUAGAcagucGgGCGAGG-CGUGCGa -3' miRNA: 3'- ugCUGGGGUCU-----CgUGCUCCgGUACGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 19872 | 0.67 | 0.308332 |
Target: 5'- aGCGcCCCaCGGAGCGCGGaaguGGCCGcggacGCGg -3' miRNA: 3'- -UGCuGGG-GUCUCGUGCU----CCGGUa----CGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 11809 | 0.67 | 0.308332 |
Target: 5'- -gGGCCCCAGAGCGagGAGcGCgUcgGCGc -3' miRNA: 3'- ugCUGGGGUCUCGUg-CUC-CG-GuaCGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 26785 | 0.67 | 0.300663 |
Target: 5'- gUGGCCCCGcGcAGCuACG-GGCuCAUGCAa -3' miRNA: 3'- uGCUGGGGU-C-UCG-UGCuCCG-GUACGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 23398 | 0.67 | 0.285768 |
Target: 5'- gGCGGCCuCCAaGGCGCGccGGCUcgGCu -3' miRNA: 3'- -UGCUGG-GGUcUCGUGCu-CCGGuaCGu -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 25047 | 0.68 | 0.271461 |
Target: 5'- cUGGCCCCGG-GCGCGccGGCCAcccaGCGc -3' miRNA: 3'- uGCUGGGGUCuCGUGCu-CCGGUa---CGU- -5' |
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87 | 5' | -59.4 | AC_000006.1 | + | 31008 | 1.08 | 0.00023 |
Target: 5'- cACGACCCCAGAGCACGAGGCCAUGCAg -3' miRNA: 3'- -UGCUGGGGUCUCGUGCUCCGGUACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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