Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8704 | 5' | -53.5 | NC_002229.2 | + | 351 | 0.69 | 0.903799 |
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Target: 5'- gCUGUCUGCGCguaGCUAUuggCCGGgcugunugCCg -3' miRNA: 3'- gGACGGACGUGa--UGAUGua-GGCCa-------GG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 1317 | 0.96 | 0.043825 |
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Target: 5'- aCCUGCCUGCAUUACUACAUCCaGUUCg -3' miRNA: 3'- -GGACGGACGUGAUGAUGUAGGcCAGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 43548 | 0.68 | 0.950635 |
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Target: 5'- aCgGCCUaGCGCUACaaaAUaCUGGUCCg -3' miRNA: 3'- gGaCGGA-CGUGAUGaugUA-GGCCAGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 47930 | 0.71 | 0.846425 |
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Target: 5'- cCCUGCCgGgACaAUgGCAUCCGG-CCa -3' miRNA: 3'- -GGACGGaCgUGaUGaUGUAGGCCaGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 60754 | 0.67 | 0.958451 |
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Target: 5'- gCUGCuguaucgagCUGCACUGCUAUagAUgCGGaaUCCg -3' miRNA: 3'- gGACG---------GACGUGAUGAUG--UAgGCC--AGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 137394 | 0.96 | 0.043825 |
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Target: 5'- aCCUGCCUGCAUUACUACAUCCaGUUCg -3' miRNA: 3'- -GGACGGACGUGAUGAUGUAGGcCAGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 138361 | 0.69 | 0.903799 |
|
Target: 5'- gCUGUCUGCGCguaGCUAUuggCCGGgcugunugCCg -3' miRNA: 3'- gGACGGACGUGa--UGAUGua-GGCCa-------GG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 138871 | 1.15 | 0.002402 |
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Target: 5'- aCCUGCCUGCACUACUACAUCCGGUCCg -3' miRNA: 3'- -GGACGGACGUGAUGAUGUAGGCCAGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 154787 | 0.66 | 0.979054 |
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Target: 5'- aUCUGCCgggUGUACUACU-CAUUugaggUGGUUCg -3' miRNA: 3'- -GGACGG---ACGUGAUGAuGUAG-----GCCAGG- -5' |
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| 8704 | 5' | -53.5 | NC_002229.2 | + | 173743 | 1.15 | 0.002402 |
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Target: 5'- aCCUGCCUGCACUACUACAUCCGGUCCg -3' miRNA: 3'- -GGACGGACGUGAUGAUGUAGGCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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