Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8723 | 3' | -56.5 | NC_002331.1 | + | 55878 | 0.67 | 0.893336 |
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Target: 5'- --aCUG-UGAaGGGUACCGCuaucCCGGCu -3' miRNA: 3'- ccaGACaACUaCCCGUGGUGc---GGCCG- -5' |
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| 8723 | 3' | -56.5 | NC_002331.1 | + | 158338 | 0.68 | 0.849706 |
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Target: 5'- gGGUCUGagGGcuuGGCuaugacaucuugaGCCACGUCGGCc -3' miRNA: 3'- -CCAGACaaCUac-CCG-------------UGGUGCGGCCG- -5' |
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| 8723 | 3' | -56.5 | NC_002331.1 | + | 5068 | 0.69 | 0.79199 |
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Target: 5'- cGGUCguagUGUUGGUGGGUugcAUCGCGUCGu- -3' miRNA: 3'- -CCAG----ACAACUACCCG---UGGUGCGGCcg -5' |
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| 8723 | 3' | -56.5 | NC_002331.1 | + | 48103 | 0.73 | 0.549038 |
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Target: 5'- cGGUCUcaUGAgcUGGGCGuucgagggaauuauaCGCGCCGGCg -3' miRNA: 3'- -CCAGAcaACU--ACCCGUg--------------GUGCGGCCG- -5' |
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| 8723 | 3' | -56.5 | NC_002331.1 | + | 72783 | 0.74 | 0.538302 |
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Target: 5'- cGGUCUaucGUaauuGUGGGaCGCCAUGUCGGCg -3' miRNA: 3'- -CCAGA---CAac--UACCC-GUGGUGCGGCCG- -5' |
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| 8723 | 3' | -56.5 | NC_002331.1 | + | 44339 | 1.13 | 0.001816 |
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Target: 5'- cGGUCUGUUGAUGGGCACCACGCCGGCc -3' miRNA: 3'- -CCAGACAACUACCCGUGGUGCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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