miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8723 3' -56.5 NC_002331.1 + 55878 0.67 0.893336
Target:  5'- --aCUG-UGAaGGGUACCGCuaucCCGGCu -3'
miRNA:   3'- ccaGACaACUaCCCGUGGUGc---GGCCG- -5'
8723 3' -56.5 NC_002331.1 + 158338 0.68 0.849706
Target:  5'- gGGUCUGagGGcuuGGCuaugacaucuugaGCCACGUCGGCc -3'
miRNA:   3'- -CCAGACaaCUac-CCG-------------UGGUGCGGCCG- -5'
8723 3' -56.5 NC_002331.1 + 5068 0.69 0.79199
Target:  5'- cGGUCguagUGUUGGUGGGUugcAUCGCGUCGu- -3'
miRNA:   3'- -CCAG----ACAACUACCCG---UGGUGCGGCcg -5'
8723 3' -56.5 NC_002331.1 + 48103 0.73 0.549038
Target:  5'- cGGUCUcaUGAgcUGGGCGuucgagggaauuauaCGCGCCGGCg -3'
miRNA:   3'- -CCAGAcaACU--ACCCGUg--------------GUGCGGCCG- -5'
8723 3' -56.5 NC_002331.1 + 72783 0.74 0.538302
Target:  5'- cGGUCUaucGUaauuGUGGGaCGCCAUGUCGGCg -3'
miRNA:   3'- -CCAGA---CAac--UACCC-GUGGUGCGGCCG- -5'
8723 3' -56.5 NC_002331.1 + 44339 1.13 0.001816
Target:  5'- cGGUCUGUUGAUGGGCACCACGCCGGCc -3'
miRNA:   3'- -CCAGACAACUACCCGUGGUGCGGCCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.