Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8723 | 5' | -53.5 | NC_002331.1 | + | 90134 | 0.66 | 0.972253 |
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Target: 5'- aUCGCCA--GAGUauuuaacuucguucgCGCCCUC-CAGUAa -3' miRNA: 3'- -AGCGGUugCUCA---------------GUGGGAGuGUCAUg -5' |
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| 8723 | 5' | -53.5 | NC_002331.1 | + | 66033 | 0.67 | 0.95794 |
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Target: 5'- -aGCCugguACGAGUCACCgCU-GCcGUGCa -3' miRNA: 3'- agCGGu---UGCUCAGUGG-GAgUGuCAUG- -5' |
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| 8723 | 5' | -53.5 | NC_002331.1 | + | 145758 | 0.68 | 0.941139 |
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Target: 5'- aCGCCAugcgcAUGGGUCugCCgUACGcGUACg -3' miRNA: 3'- aGCGGU-----UGCUCAGugGGaGUGU-CAUG- -5' |
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| 8723 | 5' | -53.5 | NC_002331.1 | + | 7837 | 0.7 | 0.867801 |
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Target: 5'- uUCGCCAGCGAGaacaaugucuaUCcaGCCUUggcgcaugCGCAGUACa -3' miRNA: 3'- -AGCGGUUGCUC-----------AG--UGGGA--------GUGUCAUG- -5' |
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| 8723 | 5' | -53.5 | NC_002331.1 | + | 13000 | 0.73 | 0.705268 |
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Target: 5'- aUGCCGACGAGUU-UCCUCGCAGc-- -3' miRNA: 3'- aGCGGUUGCUCAGuGGGAGUGUCaug -5' |
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| 8723 | 5' | -53.5 | NC_002331.1 | + | 44305 | 1.11 | 0.004118 |
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Target: 5'- aUCGCCAACGAGUCACCCUCACAGUACa -3' miRNA: 3'- -AGCGGUUGCUCAGUGGGAGUGUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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