Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8724 | 3' | -55.4 | NC_002331.1 | + | 153256 | 0.67 | 0.901545 |
Target: 5'- aUCCAauuuUGACCGauccucagcCACGGCGGC-AGCAc -3' miRNA: 3'- -AGGUc---AUUGGU---------GUGCCGCCGaUCGUc -5' |
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8724 | 3' | -55.4 | NC_002331.1 | + | 178308 | 0.69 | 0.801076 |
Target: 5'- gUCgAGUGuugucaAUgGCACGGCGGgUGGUAGa -3' miRNA: 3'- -AGgUCAU------UGgUGUGCCGCCgAUCGUC- -5' |
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8724 | 3' | -55.4 | NC_002331.1 | + | 95554 | 0.72 | 0.675314 |
Target: 5'- aCUAGUAcACCaaGCAUGGUGGCgugGGCGGc -3' miRNA: 3'- aGGUCAU-UGG--UGUGCCGCCGa--UCGUC- -5' |
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8724 | 3' | -55.4 | NC_002331.1 | + | 45057 | 1.08 | 0.003966 |
Target: 5'- uUCCAGUAACCACACGGCGGCUAGCAGc -3' miRNA: 3'- -AGGUCAUUGGUGUGCCGCCGAUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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