Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8724 | 5' | -56.1 | NC_002331.1 | + | 9258 | 0.66 | 0.93827 |
Target: 5'- cGGCCAau-CCGUCGuCGGGUAA--UGAg -3' miRNA: 3'- -CCGGUgguGGCGGU-GCCCAUUucACU- -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 137324 | 0.66 | 0.93827 |
Target: 5'- uGCCACC-CCG-CAUGGGUuGAGg-- -3' miRNA: 3'- cCGGUGGuGGCgGUGCCCAuUUCacu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 97208 | 0.66 | 0.933448 |
Target: 5'- aGUCGCCAUguaCGgCAgaGGGUGGAGUGGu -3' miRNA: 3'- cCGGUGGUG---GCgGUg-CCCAUUUCACU- -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 101050 | 0.66 | 0.933448 |
Target: 5'- uGGCagUUugCGCCAUGGGUGGGGa-- -3' miRNA: 3'- -CCGguGGugGCGGUGCCCAUUUCacu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 96828 | 0.67 | 0.905871 |
Target: 5'- uGGCCAaaAUUGCCAC-GGUAAGGUu- -3' miRNA: 3'- -CCGGUggUGGCGGUGcCCAUUUCAcu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 146106 | 0.67 | 0.899667 |
Target: 5'- uGGUCAUCACCGCCAgauuuUGGG----GUGGc -3' miRNA: 3'- -CCGGUGGUGGCGGU-----GCCCauuuCACU- -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 71031 | 0.68 | 0.85017 |
Target: 5'- uGCCACCGCCuugagagauaacGCUGCGGGUAuuaauacGUGu -3' miRNA: 3'- cCGGUGGUGG------------CGGUGCCCAUuu-----CACu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 55209 | 0.69 | 0.800249 |
Target: 5'- gGGCU--CAUCGCUAUGGGUucaAAGGUGAc -3' miRNA: 3'- -CCGGugGUGGCGGUGCCCA---UUUCACU- -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 9312 | 0.69 | 0.800249 |
Target: 5'- --gCACCGCCGcCCAUGGcG-AAAGUGAu -3' miRNA: 3'- ccgGUGGUGGC-GGUGCC-CaUUUCACU- -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 21503 | 0.7 | 0.735461 |
Target: 5'- cGCCACCACCGCCACcacGcAGAGUc- -3' miRNA: 3'- cCGGUGGUGGCGGUGcc-CaUUUCAcu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 72623 | 0.76 | 0.416519 |
Target: 5'- -aCCACCACCGCCuGCGGGUAGGa--- -3' miRNA: 3'- ccGGUGGUGGCGG-UGCCCAUUUcacu -5' |
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8724 | 5' | -56.1 | NC_002331.1 | + | 45022 | 1.03 | 0.00958 |
Target: 5'- uGGCCACCACCGCCACGGGU-AAGUGAa -3' miRNA: 3'- -CCGGUGGUGGCGGUGCCCAuUUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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